Generation of an equine biobank to be used for Functional Annotation of Animal Genomes project.
Generation of an equine biobank to be used for Functional Annotation of Animal Genomes project.
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DOI：
10.1111/age.12717
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发表时间：
2018-12
期刊：
影响因子：
2.4
通讯作者：
Finno CJ
中科院分区：
文献类型：
--
作者：
Burns EN;Bordbari MH;Mienaltowski MJ;Affolter VK;Barro MV;Gianino F;Gianino G;Giulotto E;Kalbfleisch TS;Katzman SA;Lassaline M;Leeb T;Mack M;Müller EJ;MacLeod JN;Ming-Whitfield B;Alanis CR;Raudsepp T;Scott E;Vig S;Zhou H;Petersen JL;Bellone RR;Finno CJ
动物基因组功能注释（FAANG）项目旨在确定驯养动物中两性和多个发育阶段的基因组调控元件。这项研究代表了FAANG项目的第一阶段，马，马caballus。从两只成年纯种马中创建了80个组织样本、两个细胞系和六种体液的生物库。生前评估包括全面体格检查、跛行、眼科和神经系统评价。还进行了全血细胞计数和血清生化检查。尸检时，除组织样本外，还采集了来自胃肠道和泌尿生殖道所有区域的等份血清、乙二胺四乙酸血浆、肝素化血浆、脑脊液、滑液、尿液和微生物组样本。从皮肤样品培养表皮角质形成细胞和真皮成纤维细胞。由委员会认证的兽医病理学家对所有组织进行大体和组织学评价。临床和病理学评价结果发现，在两只动物中，整个胃肠道均存在亚临床嗜酸性粒细胞和淋巴细胞浸润以及轻度临床跛行。每个样品以多种方式冷冻保存，并从选定的组织中提取细胞核。这些样本代表了第一个发表的全身健康的马特异性生物样本库，具有广泛的临床表型的生前和死后。生物库中的组织旨在用于马基因组的功能注释。生物库的使用将提高参考注释的质量，并允许所有马的研究人员阐明未知的基因组和表观基因组疾病的原因。
The Functional Annotation of Animal Genomes (FAANG) project aims to identify genomic regulatory elements in both sexes and across multiple stages of development in domesticated animals. This study represents the first stage of the FAANG project for the horse, Equus caballus. A biobank of 80 tissue samples, two cell lines and six body fluids was created from two adult Thoroughbred mares. Ante-mortem assessments included full physical examinations, lameness, ophthalmologic and neurologic evaluations. Complete blood counts and serum biochemistries were also performed. At necropsy, in addition to tissue samples, aliquots of serum, ethylenediaminetetraacetic acid plasma, heparinized plasma, cerebrospinal fluid, synovial fluid, urine and microbiome samples from all regions of the gastrointestinal and urogenital tracts were collected. Epidermal keratinocytes and dermal fibroblasts were cultured from skin samples. All tissues were grossly and histologically evaluated by a board-certified veterinary pathologist. The results of the clinical and pathological evaluations identified subclinical eosinophilic and lymphocytic infiltration throughout the length of the gastrointestinal tract as well as a mild clinical lameness in both animals. Each sample was cryo-preserved in multiple ways, and nuclei were extracted from selected tissues. These samples represent the first published systemically healthy equine-specific biobank with extensive clinical phenotyping ante- and post-mortem. The tissues in the biobank are intended for community-wide use in the functional annotation of the equine genome. The use of the biobank will improve the quality of the reference annotation and allow all equine researchers to elucidate unknown genomic and epigenomic causes of disease.
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影响因子：
3.7
作者：
Brady, Rebecca A.;Bruno, Vincent M.;Burns, Drusilla L.
通讯作者：
Burns, Drusilla L.
影响因子：
12.3
作者：
Andersson L;Archibald AL;Bottema CD;Brauning R;Burgess SC;Burt DW;Casas E;Cheng HH;Clarke L;Couldrey C;Dalrymple BP;Elsik CG;Foissac S;Giuffra E;Groenen MA;Hayes BJ;Huang LS;Khatib H;Kijas JW;Kim H;Lunney JK;McCarthy FM;McEwan JC;Moore S;Nanduri B;Notredame C;Palti Y;Plastow GS;Reecy JM;Rohrer GA;Sarropoulou E;Schmidt CJ;Silverstein J;Tellam RL;Tixier-Boichard M;Tosser-Klopp G;Tuggle CK;Vilkki J;White SN;Zhao S;Zhou H;FAANG Consortium
通讯作者：
FAANG Consortium
影响因子：
13.6
作者：
Ronningen, Kjersti S.;Paltiel, Liv;Hoppin, Jane A.
通讯作者：
Hoppin, Jane A.
影响因子：
1.5
作者：
Albl, Barbara;Haesner, Serena;Blutke, Andreas
通讯作者：
Blutke, Andreas
影响因子：
1.6
作者：
Milley, Kristi M.;Nimmo, Judith S.;Danks, Janine A.
通讯作者：
Danks, Janine A.