Enteroviral RNA sequences detected by polymerase chain reaction in muscle of patients with postviral fatigue syndrome.
Enteroviral RNA sequences detected by polymerase chain reaction in muscle of patients with postviral fatigue syndrome.
复制标题
通过聚合酶链反应检测病毒后疲劳综合征患者肌肉中的肠道病毒 RNA 序列。
DOI：
10.1136/bmj.302.6778.692
复制
发表时间：
1991
影响因子：
--
通讯作者：
P. Behan
中科院分区：
文献类型：
--
作者：
J. Gow;W. Behan;G. Clements;C. Woodall;M. Riding;P. Behan
目的--确定病毒感染后疲劳综合征患者肌肉中肠道病毒序列的存在。设计--在一组明确定义的综合征患者和同期对照组中，用聚合酶链反应检测序列。地点--格拉斯哥神经科学研究所。受试者--60名连续的病毒感染后疲劳综合征患者，他们接受了广泛的调查以排除其他疾病。来自同一集水区的41名对照，无疲劳迹象，均接受常规手术。主要观察指标--常规检查、针对一系列病毒的抗体的血清学筛查以及肌肉活检标本中肠道病毒RNA序列的存在。结果：15例（25%）患者和10例（24.4%）对照组有重要的血清学发现。12例患者的柯萨奇病毒B1-5中和抗体滴度大于或等于256（6例肠道病毒RNA序列阳性，6例阴性）; 3例EB病毒特异性IgM阳性（2例阳性，1例阴性）。6名对照组的柯萨奇病毒中和抗体滴度相似（均为阴性）; 1名对照组的EB病毒特异性IgM阳性（阴性）; 3名对照组的巨细胞病毒补体结合抗体滴度大于或等于256（均为阴性）。总体而言，肌肉中肠道病毒RNA序列的患者显著多于对照组（32/60，53% vs 6/41，15%;比值比6.7，95%置信区间2.4 - 18.2）。这与病程、患者和年龄无关，也与柯萨奇病毒B1-5抗体滴度升高无关。结论--在一些病毒后疲劳综合征患者中可能发生持续性肠道病毒肌肉感染，并可能具有病原学作用。
OBJECTIVE--To determine the presence of enteroviral sequences in muscle of patients with the postviral fatigue syndrome. DESIGN--Detection of sequences with the polymerase chain reaction in a well defined group of patients with the syndrome and controls over the same period. SETTING--Institute of Neurological Sciences, Glasgow. SUBJECTS--60 consecutive patients admitted to the institute with the postviral fatigue syndrome who had undergone extensive investigation to exclude other conditions. 41 controls from the same catchment area without evidence of fatigue, all undergoing routine surgery. MAIN OUTCOME MEASURES--Routine investigations, serological screen for antibodies to a range of viruses, and presence of enteroviral RNA sequences in muscle biopsy specimens. RESULTS--15 (25%) patients and 10 (24.4%) controls had important serological findings. 12 patients had neutralising antibody titres of greater than or equal to 256 to coxsackieviruses B1-5 (six positive for enteroviral RNA sequences, six negative); three were positive for Epstein-Barr virus specific IgM (two positive, one negative). Six controls had similar neutralising antibody titres to coxsackieviruses (all negative); one was positive for Epstein-Barr virus specific IgM (negative); and three had titres of complement fixing antibody greater than or equal to 256 to cytomegalovirus (all negative). Overall, significantly more patients than controls had enteroviral RNA sequences in muscle (32/60, 53% v 6/41, 15%; odds ratio 6.7, 95% confidence interval 2.4 to 18.2). This was not correlated with duration of disease, patient and age, or to raised titres of antibodies to coxsackieviruses B1-5. CONCLUSIONS--Persistent enteroviral infection of muscle may occur in some patients with postviral fatigue syndrome and may have an aetiological role.
DOI：
10.1056/nejm199001183220307
发表时间：
1990-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
B. Eisenstein
通讯作者：
B. Eisenstein
在没有细胞损伤的形态学证据的情况下，病毒可以引起疾病：对未来发现新疾病的启示。
DOI：
10.1093/infdis/159.3.384
发表时间：
1989
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Oldstone,MB
通讯作者：
Oldstone,MB