Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection.
Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection.
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舌鳎（Cynoglossus semilaevis）脾脏中巨细胞病毒诱导蛋白的整体分析鉴定了病毒感染所必需的细胞过程
DOI：
10.1016/j.dci.2018.11.006
复制
发表时间：
2019-03
影响因子：
2.9
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Zhang J;Sun L
巨细胞病毒是一种DNA病毒，在包括半滑舌鳎（Cynoglossus semilaevis）在内的养殖鱼类中具有广泛的宿主范围。在这项研究中，无标记蛋白质组学进行检测蛋白质表达的巨细胞病毒感染后8和12天（dpi）诱导的舌鞋底脾。与未感染对照鱼相比，病毒感染鱼在8 dpi时显示出315个上调蛋白和111个下调蛋白，在12 dpi时显示出48个上调蛋白和43个下调蛋白。Western印迹证实了五种差异表达蛋白的表达。差异表达蛋白主要集中在与先天免疫应答和病毒感染相关的途径和过程中。干扰泛素蛋白酶体系统（UPS），即蛋白酶体组装伴侣蛋白2和蛋白酶体成熟蛋白的两个上调蛋白的表达，显着减少病毒在鱼中的繁殖，而这两种蛋白的过表达显着增强病毒的繁殖。一致地，蛋白酶体功能的抑制显著损害病毒在体内的复制。这项研究提供了第一个全球性的蛋白质谱响应巨细胞病毒在舌鳎，并揭示了UPS在病毒感染中的重要作用。对巨细胞病毒感染的滑舌鳎的蛋白质表达谱进行了研究。共鉴定出517个差异表达蛋白（DEPs）。DEP在免疫应答和病毒感染途径中富集。泛素蛋白酶体系统（UPS）因子是DEP之一。UPS的正常表达和功能是病毒感染所必需的。
Megalocytivirus is a DNA virus with a broad host range among farmed fish including tongue sole (Cynoglossus semilaevis). In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi). Compared to uninfected control fish, virus-infected fish displayed 315 up-regulated proteins and 111 down-regulated proteins at 8 dpi, and 48 up-regulated proteins and 43 down-regulated proteins at 12 dpi. The expressions of five differentially expressed proteins were confirmed by Western blot. The differentially expressed proteins were enriched in the pathways and processes associated with innate immune response and viral infection. Interference with the expression of two up-regulated proteins of the ubiquitin proteasome system (UPS), i.e. proteasome assembly chaperone 2 and proteasome maturation protein, significantly reduced viral propagation in fish, whereas overexpression of these two proteins significantly enhanced viral propagation. Consistently, inhibition of the functioning of proteasome significantly impaired viral replication in vivo. This study provided the first global protein profile responsive to megalocytivirus in tongue sole, and revealed an essential role of UPS in viral infection. The protein expression profile in megalocytivirus-infected tongue sole was investigated. A total of 517 differentially expressed proteins (DEPs) were identified. The DEPs were enriched in pathways of immune response and viral infection. Factors of the ubiquitin proteasome system (UPS) were among the DEPs. Normal expression and functioning of the UPS were essential to viral infection.
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DOI：
10.3390/v4071075
发表时间：
2012-07
期刊：
Viruses
影响因子：
--
作者：
Grayfer L;Andino Fde J;Chen G;Chinchar GV;Robert J
通讯作者：
Robert J
影响因子：
5.4
作者：
Guo, Chang-Jun;Wu, Yan-Yan;He, Jian-Guo
通讯作者：
He, Jian-Guo
DOI：
10.1073/pnas.0406821101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Feire, AL;Koss, H;Compton, T
通讯作者：
Compton, T
鳜鱼的核心泛素系统可被传染性脾肾坏死病毒利用
影响因子：
4.7
作者：
Huang, Runqing;Zhang, Jing;Xie, Junfeng
通讯作者：
Xie, Junfeng
影响因子：
3.7
作者：
Caipang, CMA;Hirono, I;Aoki, T
通讯作者：
Aoki, T