Taurine Protects against the Fatty Liver Hemorrhagic Syndrome in Laying Hens through the Regulation of Mitochondrial Homeostasis.
Taurine Protects against the Fatty Liver Hemorrhagic Syndrome in Laying Hens through the Regulation of Mitochondrial Homeostasis.
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DOI：
10.3390/ijms241210360
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发表时间：
2023-06-20
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
代谢相关脂肪性肝病(MAFLD)是一种由人和哺乳动物肝脏脂肪沉积引起的慢性肝病，而脂肪肝出血综合征(FlHS)是蛋鸡的一种脂肪性肝病，可增加蛋鸡的死亡率，给蛋鸡养殖业造成严重的经济损失。越来越多的证据表明，脂肪肝的发生与线粒体动态平衡的破坏密切相关。研究证明，牛磺酸可以调节肝脏脂肪代谢，减少肝脏脂肪沉积，抑制氧化应激，缓解线粒体功能障碍。然而，牛磺酸调节肝细胞线粒体动态平衡的机制还有待进一步研究。在本研究中，我们研究了牛磺酸对高能低蛋白饲料诱导的蛋鸡和培养的肝细胞中游离脂肪酸(FFA)诱导的脂肪变性的影响及其机制。检测肝功能、脂质代谢、抗氧化能力、线粒体功能、线粒体动力学、自噬和生物合成。结果表明，在FLHS母鸡和脂肪变性肝细胞中，肝脏结构和功能受损，线粒体损伤和功能障碍，脂质堆积，线粒体融合和分裂之间的失衡，线粒体自噬和生物合成。牛磺酸能显著抑制肝纤维化的发生，保护线粒体免受脂质积聚和游离脂肪酸所致的损伤，上调Mfn1、Mfn2、OPA1、Lc3I、Lc3II、PINK1、PGC-1α、Nrf1、Nrf2和Tfam的表达水平，下调Fis1、DRp1和p62的表达水平。综上所述，牛磺酸可以通过调节线粒体动态平衡，包括调节线粒体动力学、自噬和生物合成来保护蛋鸡免受FLHS的侵袭。
Metabolic-associated fatty liver disease (MAFLD) is a chronic liver disease caused by fat deposition in the liver of humans and mammals, while fatty liver hemorrhagic syndrome (FLHS) is a fatty liver disease in laying hens which can increase the mortality and cause severe economic losses to the laying industry. Increasing evidence has shown a close relationship between the occurrence of fatty liver disease and the disruption of mitochondrial homeostasis. Studies have proven that taurine can regulate hepatic fat metabolism, reduce hepatic fatty deposition, inhibit oxidative stress, and alleviate mitochondrial dysfunction. However, the mechanisms by which taurine regulates mitochondrial homeostasis in hepatocytes need to be further studied. In this study, we determined the effects and mechanisms of taurine on high-energy low-protein diet-induced FLHS in laying hens and in cultured hepatocytes in free fatty acid (FFA)-induced steatosis. The liver function, lipid metabolism, antioxidant capacity, mitochondrial function, mitochondrial dynamics, autophagy, and biosynthesis were detected. The results showed impaired liver structure and function, mitochondrial damage and dysfunction, lipid accumulation, and imbalance between mitochondrial fusion and fission, mitochondrial autophagy, and biosynthesis in both FLHS hens and steatosis hepatocytes. Taurine administration can significantly inhibit the occurrence of FLHS, protect mitochondria in hepatocytes from disease induced by lipid accumulation and FFA, up-regulate the expression levels of Mfn1, Mfn2, Opa1, LC3I, LC3II, PINK1, PGC-1α, Nrf1, Nrf2, and Tfam, and down-regulate the expression levels of Fis1, Drp1, and p62. In conclusion, taurine can protect laying hens from FLHS through the regulation of mitochondrial homeostasis, including the regulation of mitochondrial dynamics, autophagy, and biosynthesis.
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影响因子：
4
作者：
Guo L;Kuang J;Zhuang Y;Jiang J;Shi Y;Huang C;Zhou C;Xu P;Liu P;Wu C;Hu G;Guo X
通讯作者：
Guo X
影响因子：
82.9
作者：
Friedman SL;Neuschwander-Tetri BA;Rinella M;Sanyal AJ
通讯作者：
Sanyal AJ
影响因子：
6.1
作者：
Chang, Yuan-Yen;Chou, Chung-Hsi;Chen, Yi-Chen
通讯作者：
Chen, Yi-Chen
DOI：
10.1152/ajpregu.00677.2010
发表时间：
2011-12-01
影响因子：
2.8
作者：
Gentile, Christopher L.;Nivala, Angela M.;Maclean, Kenneth N.
通讯作者：
Maclean, Kenneth N.
影响因子：
3.4
作者：
Goncalves, Ines O.;Passos, Emanuel;Magalhaes, Jose
通讯作者：
Magalhaes, Jose