Serum Metabolomic Profiling to Reveal Potential Biomarkers for the Diagnosis of Fatty Liver Hemorrhagic Syndrome in Laying Hens.
Serum Metabolomic Profiling to Reveal Potential Biomarkers for the Diagnosis of Fatty Liver Hemorrhagic Syndrome in Laying Hens.
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DOI：
10.3389/fphys.2021.590638
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发表时间：
2021
影响因子：
4
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Guo L;Kuang J;Zhuang Y;Jiang J;Shi Y;Huang C;Zhou C;Xu P;Liu P;Wu C;Hu G;Guo X
脂肪肝出血综合征是产蛋鸡常见的营养代谢性疾病，给养禽业造成严重损失。目前，FLHS的传统临床诊断仍有其局限性。因此，寻找一些代谢生物标志物，阐明体内代谢途径，对FLHS的诊断和预防有重要意义。本研究采用高能低蛋白日粮诱导产蛋鸡FLHS模型。采用气相色谱-飞行时间质谱法（GC-TOF-MS）分析第40天和第80天血清中的代谢物。结果表明，共筛选鉴定出40种与FLHS发生发展密切相关的差异代谢产物，主要与脂质代谢、氨基酸代谢和能量代谢途径紊乱有关。进一步研究差异代谢产物，通过受试者工作特征（ROC）曲线分析，发现3-羟基丁酸、油酸、棕榈油酸、谷氨酸等10种潜在生物标志物具有较高的诊断价值。本研究表明，基于GC-TOF-MS的代谢组学方法可用于蛋鸡FLHS的临床诊断，并为FLHS的早期风险评估和进一步了解FLHS的发展提供潜在的生物标志物。
Fatty liver hemorrhage syndrome (FLHS), a nutritional and metabolic disease that frequently occurs in laying hens, causes serious losses to the poultry industry. Nowadays, the traditional clinical diagnosis of FLHS still has its limitations. Therefore, searching for some metabolic biomarkers and elucidating the metabolic pathway in vivo are useful for the diagnosis and prevention of FLHS. In the present study, a model of FLHS in laying hens induced by feeding a high-energy, low-protein diet was established. Gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) was used to analyze the metabolites in serum at days 40 and 80. The result showed that, in total, 40 differential metabolites closely related to the occurrence and development of FLHS were screened and identified, which were mainly associated with lipid metabolism, amino acid metabolism, and energy metabolism pathway disorders. Further investigation of differential metabolites showed 10 potential biomarkers such as 3-hydroxybutyric acid, oleic acid, palmitoleic acid, and glutamate were possessed of high diagnostic values by analyzing receiver operating characteristic (ROC) curves. In conclusion, this study showed that the metabolomic method based on GC-TOF-MS can be used in the clinical diagnosis of FLHS in laying hens and provide potential biomarkers for early risk evaluation of FLHS and further insights into FLHS development.
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影响因子：
5.3
作者：
Arrol, S;Mackness, MI;Durrington, PN
通讯作者：
Durrington, PN
影响因子：
5.6
作者：
Gambino R;Bugianesi E;Rosso C;Mezzabotta L;Pinach S;Alemanno N;Saba F;Cassader M
通讯作者：
Cassader M
影响因子：
5.2
作者：
Ismail, Israa T.;Fiehn, Oliver;El-Said, Hala
通讯作者：
El-Said, Hala
中国轻度持续性哮喘患者血清代谢变化：基于 GC-MS 的代谢组学分析
影响因子：
8.2
作者：
Chang, Chun;Guo, Zhi-guo;Yao, Wan-zhen
通讯作者：
Yao, Wan-zhen
DOI：
10.1080/03079457608418164
发表时间：
1976-01-01
期刊：
Avian pathology : journal of the W.V.P.A
影响因子：
--
作者：
Butler, E J
通讯作者：
Butler, E J