Toward understanding the role of cartilage particulates in synovial inflammation.
Toward understanding the role of cartilage particulates in synovial inflammation.
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DOI：
10.1016/j.joca.2017.03.015
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发表时间：
2017-08
影响因子：
7
通讯作者：
Hung CT
中科院分区：
文献类型：
--
作者：
Silverstein AM;Stefani RM;Sobczak E;Tong EL;Attur MG;Shah RP;Bulinski JC;Ateshian GA;Hung CT
关节镜下灌洗和滑膜切除术可以清除关节间隙和滑膜内层的组织碎片，以缓解骨关节炎（OA）患者的疼痛。在这里，我们开发了一种体外模型来研究软骨磨损颗粒与成纤维细胞样滑膜细胞（FLS）的相互作用，以更好地了解软骨颗粒与滑膜细胞上的细胞因子的相互作用。本研究将10μm以下的软骨颗粒或1μm的乳胶颗粒与FLS ± 10 ng/mL的白细胞介素-1 α（IL-1 α）或肿瘤坏死因子- α（TNF-α）共培养。分析样品的DNA、糖胺聚糖（GAG）和胶原蛋白，并分析培养基样品的培养基GAG、一氧化氮（NO）和前列腺素-E2（PGE 2）。颗粒和FLS之间的物理相互作用的性质通过显微镜测定。乳胶和软骨颗粒均可被FLS吞噬。软骨素颗粒被内化并附着在致密单层和单个细胞的表面。与对照组和乳胶处理组相比，FLS与软骨颗粒的共培养导致细胞片层DNA和胶原含量以及NO和PGE 2合成的显著增加。FLS对软骨磨损颗粒的增殖反应导致ECM含量的总体增加，类似于在OA患者中观察到的滑膜衬里增厚。了解软骨颗粒与滑膜的相互作用可能有助于了解这种相互作用如何促进OA进展，并可能指导灌洗和滑膜切除术在退行性疾病中的作用。
Arthroscopy with lavage and synovectomy can remove tissue debris from the joint space and the synovial lining to provide pain relief to patients with osteoarthritis (OA). Here, we developed an in vitro model to study the interaction of cartilage wear particles with fibroblast-like synoviocytes (FLS) to better understand the interplay of cartilage particulates with cytokines on cells of the synovium. In this study sub-10μm cartilage particles or 1μm latex particles were co-cultured with FLS ± 10 ng/mL interleukin-1α (IL-1 α) or tumor necrosis factor- α (TNF-α). Samples were analyzed for DNA, glycosaminoglycan (GAG), and collagen and media samples were analyzed for media GAG, nitric oxide (NO) and prostaglandin-E2 (PGE2). The nature of the physical interaction between the particles and FLS was determined by microscopy. Both latex and cartilage particles could be phagocytosed by FLS. Cartilage particles were internalized and attached to the surface of both dense monolayers and individual cells. Co-culture of FLS with cartilage particulates resulted in a significant increase in cell sheet DNA and collagen content as well as NO and PGE2 synthesis compared to control and latex treated groups. The proliferative response of FLS to cartilage wear particles resulted in an overall increase in ECM content, analogous to the thickening of the synovial lining observed in OA patients. Understanding how cartilage particles interface with the synovium may provide insight into how this interaction contributes to OA progression and may guide the role of lavage and synovectomy for degenerative disease.
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DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
--
作者：
EVANS, CH;MEARS, DC;MCKNIGHT, JL
通讯作者：
MCKNIGHT, JL
影响因子：
3.8
作者：
Blewis, Megan E.;Lao, Brian J.;Sah, Robert L.
通讯作者：
Sah, Robert L.
影响因子：
4.9
作者：
Chiu, Richard;Ma, Ting;Goodman, Stuart B.
通讯作者：
Goodman, Stuart B.
DOI：
10.1016/j.berh.2009.08.011
发表时间：
2010-02-01
影响因子：
5.2
作者：
Attur, Mukundan;Samuels, Jonathan;Abramson, Steven B.
通讯作者：
Abramson, Steven B.