A comparative proteomics analysis of the egg secretions of three major schistosome species.
A comparative proteomics analysis of the egg secretions of three major schistosome species.
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DOI：
10.1016/j.molbiopara.2020.111322
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发表时间：
2020-11
影响因子：
1.5
通讯作者：
Gobert GN
中科院分区：
文献类型：
--
作者：
Carson JP;Robinson MW;Hsieh MH;Cody J;Le L;You H;McManus DP;Gobert GN
与肝和泌尿生殖道血吸虫病相关的发病主要源于宿主针对寄生虫卵的免疫应答。当卵被捕获在宿主组织中时，纤维化斑块的发展驱动下游病理学。这些事件的发生是由于鸡蛋排泄/分泌产物（ESP）的抗原性。曼氏血吸虫（Schistosomamansoni）和曼氏血吸虫（S.已经显示日本血吸虫ESP与宿主肝脏中的几种细胞群（包括肝细胞、巨噬细胞和肝星状细胞）相互作用，具有免疫调节和病理学后果。对S. mansoni和S.日本血吸虫卵的特征已被描述;然而，缺乏对寄生虫卵ESP的集体内容物的研究。利用鸟枪质谱法和一系列的计算机分析，我们确定了266，90和50蛋白在S。mansoni，S. japonicum和S.血吸虫卵分泌器。我们确定了许多蛋白质已经建立免疫调节活性，候选疫苗和囊泡标记。通过BLAST鉴定出相对较少的ESP内的常见直向同源物，表明三种卵分泌物在含量上显著不同。对这些成分有更清楚的了解可能会导致识别具有未表征的免疫调节潜力或病理相关性的新蛋白质。这将增强我们对宿主-寄生虫相互作用的理解，特别是在慢性血吸虫病期间发生的相互作用，并为新的治疗方法和疫苗铺平道路。
Morbidity associated with hepatic and urogenital schistosomiasis stems primarily from the host immune response directed against schistosome eggs. When eggs become entrapped in host tissues, the development of fibrotic plaques drives downstream pathology. These events occur due to the antigenic nature of egg excretory/secretory products (ESPs). Both Schistosoma mansoni and S. japonicum ESPs have been shown to interact with several cell populations in the host liver including hepatocytes, macrophages, and hepatic stellate cells, with both immunomodulatory and pathological consequences. Several protein components of the ESPs of S. mansoni and S. japonicum eggs have been characterised; however, studies into the collective contents of schistosome egg ESPs are lacking. Utilising shotgun mass spectrometry and an array of in silico analyses, we identified 266, 90 and 50 proteins within the S. mansoni, S. japonicum and S. haematobium egg secretomes respectively. We identified numerous proteins with already established immunomodulatory activities, vaccine candidates and vesicle markers. Relatively few common orthologues within the ESPs were identified by BLAST, indicating that the three egg secretomes differ in content significantly. Having a clearer understanding of these components may lead to the identification of new proteins with uncharacterised immunomodulatory potential or pathological relevance. This will enhance our understanding of host-parasite interactions, particularly those occurring during chronic schistosomiasis, and pave the way towards novel therapeutics and vaccines.
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影响因子：
2.4
作者：
DUNNE, DW;JONES, FM;DOENHOFF, MJ
通讯作者：
DOENHOFF, MJ
影响因子：
2.2
作者：
Kasper, G;Brown, A;Pritchard, DI
通讯作者：
Pritchard, DI
日本血吸虫卵抗原p40通过激活STAT3/p53/p21通路促进活化肝星状细胞衰老
影响因子：
9
作者：
Chen J;Xu T;Zhu D;Wang J;Huang C;Lyu L;Hu B;Sun W;Duan Y
通讯作者：
Duan Y
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M
影响因子：
3.8
作者：
Floudas A;Cluxton CD;Fahel J;Khan AR;Saunders SP;Amu S;Alcami A;Fallon PG
通讯作者：
Fallon PG