Chronic electronic cigarette use elicits molecular changes related to pulmonary pathogenesis.
Chronic electronic cigarette use elicits molecular changes related to pulmonary pathogenesis.
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长期使用电子烟会引起与肺部发病机制相关的分子变化。
DOI：
10.1016/j.taap.2020.115224
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发表时间：
2020-11-01
影响因子：
3.8
通讯作者：
Gao W
中科院分区：
文献类型：
--
作者：
Marshall K;Liu Z;Olfert IM;Gao W
The relative safety of chronic exposure to electronic cigarette (e-cig) aerosol remains unclear in terms of lung pathogenesis. Therefore, this study aims to evaluate gene/protein biomarkers, which are associated with cigarette-induced pulmonary injury in animals chronically exposed to nicotine containing e-cig aerosol. C57BL/6J mice were randomly assigned to three exposure groups: e-cig, tobacco cigarette smoke, and filtered air. Lung tissues and/or paraffin embedded slides were used to evaluate gene and/or protein expressions of the CYP450 metabolism (CYP1A1, CYP2A5, and CYP3A11), oxidative stress (Nrf2, SOD1), epithelial-mesenchymal transition (E-cadherin and vimentin), lung pathogenesis (AhR), and survival/apoptotic pathways (p-AKT, BCL-XL, p53, p21, and CRM1). Expressions of E-cadherin and CRM1 were significantly decreased, while CYP1A1, AhR, SOD1 and BCL-XL were significantly upregulated in the e-cig group compared to the control (p<0.05). Nuclear sub-cellular localization of p53, evaluated by immunohistochemistry staining, in bronchiolar tissues was higher in the e-cig group (25.3±2.7%) as compared to controls (12.1±1.8%) (p<0.01). Although the biomarkers responses were not identical, in general, the responses had similar qualitative trends between the e-cig and cigarette groups. As these related molecular changes are involved in the pathogenesis of cigarette-induced lung injury, the possibility exists that e-cigs can produce a similar outcome. Although further investigation is warranted, e-cigs are unlikely to be considered as safe in terms of pulmonary health.
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影响因子：
16.6
作者：
Choi S;Chen Z;Tang LH;Fang Y;Shin SJ;Panarelli NC;Chen YT;Li Y;Jiang X;Du YN
通讯作者：
Du YN
影响因子：
5.2
作者：
Cheng T
通讯作者：
Cheng T
影响因子：
2
作者：
Calkins, Hugh;Hindricks, Gerhard;Yamane, Teiichi
通讯作者：
Yamane, Teiichi
影响因子：
39.2
作者：
Mirbolouk, Mohammadhassan;Charkhchi, Paniz;Blaha, Michael J.
通讯作者：
Blaha, Michael J.
影响因子：
3.7
作者：
Sussan TE;Gajghate S;Thimmulappa RK;Ma J;Kim JH;Sudini K;Consolini N;Cormier SA;Lomnicki S;Hasan F;Pekosz A;Biswal S
通讯作者：
Biswal S