Exposure to electronic cigarettes impairs pulmonary anti-bacterial and anti-viral defenses in a mouse model.
Exposure to electronic cigarettes impairs pulmonary anti-bacterial and anti-viral defenses in a mouse model.
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DOI：
10.1371/journal.pone.0116861
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Biswal S
中科院分区：
文献类型：
--
作者：
Sussan TE;Gajghate S;Thimmulappa RK;Ma J;Kim JH;Sudini K;Consolini N;Cormier SA;Lomnicki S;Hasan F;Pekosz A;Biswal S
Electronic cigarettes (E-cigs) have experienced sharp increases in popularity over the past five years due to many factors, including aggressive marketing, increased restrictions on conventional cigarettes, and a perception that E-cigs are healthy alternatives to cigarettes. Despite this perception, studies on health effects in humans are extremely limited and in vivo animal models have not been generated. Presently, we determined that E-cig vapor contains 7x1011 free radicals per puff. To determine whether E-cig exposure impacts pulmonary responses in mice, we developed an inhalation chamber for E-cig exposure. Mice that were exposed to E-cig vapor contained serum cotinine concentrations that are comparable to human E-cig users. E-cig exposure for 2 weeks produced a significant increase in oxidative stress and moderate macrophage-mediated inflammation. Since, COPD patients are susceptible to bacterial and viral infections, we tested effects of E-cigs on immune response. Mice that were exposed to E-cig vapor showed significantly impaired pulmonary bacterial clearance, compared to air-exposed mice, following an intranasal infection with Streptococcus pneumonia. This defective bacterial clearance was partially due to reduced phagocytosis by alveolar macrophages from E-cig exposed mice. In response to Influenza A virus infection, E-cig exposed mice displayed increased lung viral titers and enhanced virus-induced illness and mortality. In summary, this study reports a murine model of E-cig exposure and demonstrates that E-cig exposure elicits impaired pulmonary anti-microbial defenses. Hence, E-cig exposure as an alternative to cigarette smoking must be rigorously tested in users for their effects on immune response and susceptibility to bacterial and viral infections.
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影响因子：
4.3
作者：
Razani-Boroujerdi, S;Singh, SP;Sopori, ML
通讯作者：
Sopori, ML
DOI：
10.1164/rccm.200311-1521oc
发表时间：
2004-12-01
影响因子：
24.7
作者：
Drannik, AG;Pouladi, MA;Stämpfli, MR
通讯作者：
Stämpfli, MR
影响因子：
6
作者：
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通讯作者：
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影响因子：
5.6
作者：
Mahapatra, Santanu Kar;Bhattacharjee, Surajit;Roy, Somenath
通讯作者：
Roy, Somenath
影响因子：
6.4
作者：
Berenson, Charles S.;Garlipp, Mary Alice;Sethi, Sanjay
通讯作者：
Sethi, Sanjay