Model selection and efficiency testing for normalization of cDNA microarray data.
Model selection and efficiency testing for normalization of cDNA microarray data.
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DOI:
10.1186/gb-2004-5-8-r60
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发表时间:
2004
期刊:
影响因子:
12.3
通讯作者:
Crompton T
中科院分区:
文献类型:
--
作者:
Futschik M;Crompton T
This study presents two novel normalization schemes for cDNA microarrays. They are based on iterative local regression and optimization of model parameters by generalized cross-validation. In this study we present two novel normalization schemes for cDNA microarrays. They are based on iterative local regression and optimization of model parameters by generalized cross-validation. Permutation tests assessing the efficiency of normalization demonstrated that the proposed schemes have an improved ability to remove systematic errors and to reduce variability in microarray data. The analysis also reveals that without parameter optimization local regression is frequently insufficient to remove systematic errors in microarray data.
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