Establishment of Down's syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT.
Establishment of Down's syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT.
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DOI：
10.1007/s13577-021-00621-0
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发表时间：
2022-01
期刊：
影响因子：
4.3
通讯作者：
Funatsu T
中科院分区：
文献类型：
--
作者：
Asakawa T;Yamada A;Kugino M;Hasegawa T;Yoshimura K;Sasa K;Kinoshita M;Nitta M;Nagata K;Sugiyama T;Kamijo R;Funatsu T
唐氏综合症是人类最常见的先天性遗传疾病之一，受影响的患者患牙周病的风险增加。为了研究牙周病的发展与疾病的参与，我们建立了永生化的牙周膜细胞从唐氏综合征患者使用SV 40 T-Ag和hTERT基因转染。在从健康（STPDL）和唐氏综合征患者（STPDLDS）样品建立的牙周膜细胞来源的永生化细胞中观察SV 40 T-Ag和hTERT的表达。从健康受试者（pPDL）获得的原代培养的牙周膜细胞具有有限数量的群体倍增数（< 40），而STPDL和STPDLDS细胞继续生长，具有超过80的群体倍增数。从患者中获得的原代培养的牙周膜细胞显示出具有21三体的唐氏综合征的染色体模式特征，而STPDLDS样品在这些结果中显示出大量的异常染色体。基因表达分析显示DSCR-1在STPDLDS中的表达高于STPDL。这些结果表明，新建立的STPDLDS细胞系可能是一个有用的工具，在唐氏综合征患者的牙周疾病的研究。在线版本包含补充材料，可通过10.1007/s13577-021-00621-0获得。
Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down’s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down’s syndrome patients. The online version contains supplementary material available at 10.1007/s13577-021-00621-0.
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4.1
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