Characterization of the peptidergic afferent innervation of the stomach in the rat, mouse and guinea-pig
Characterization of the peptidergic afferent innervation of the stomach in the rat, mouse and guinea-pig
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大鼠、小鼠和豚鼠胃肽能传入神经支配的表征
DOI：
10.1016/0306-4522(88)90017-6
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
G. Dockray
中科院分区：
文献类型：
--
作者：
T. Grhkn;G. Dockray
荧光标记的逆行追踪。 True Blue 已与使用 P 物质抗体、降钙素基因相关肽、生长抑素、血管活性肠多肽和吗啡调节肽的免疫组织化学一起使用，研究大鼠、小鼠和豚鼠胃的传入神经支配。所有三个物种中高达 85% 的胃部脊髓传入神经都含有免疫反应性降钙素基因相关肽。并且高达 50% 含有 P 物质。在所有三个物种中，不到 10% 的迷走神经传入胃部与降钙素基因相关肽的抗体发生反应。胃肌间丛、环肌及粘膜下血管周围发现降钙素基因相关肽免疫纤维。在大鼠中，去除尾肠神经节、内脏神经切片或辣椒素治疗实际上消除了胃中降钙素基因相关肽的免疫反应性。辣椒素和内脏神经切片也消除了腹腔神经节中免疫反应性降钙素基因相关肽纤维的染色。相同的治疗消除了粘膜下血管周围纤维的 P 物质染色，但在肌间神经丛和环形平滑肌中仍然存在丰富的免疫反应性纤维，可能是由内在细胞体产生的。尽管生长抑素定位于大鼠背根神经节的细胞体，但未发现含有生长抑素的内脏传入神经。未发现含有免疫反应性血管活性肠多肽的背根神经节神经元：尽管吗啡调节肽抗体显示出免疫反应性神经细胞体，但我们无法排除该结果可归因于与降钙素基因相关肽交叉反应的可能性。这些结果提供了直接证据，表明降钙素基因相关肽是内脏初级传入神经元主要亚群的标记物，并表明该群体的脊髓传入神经元是内脏初级传入神经元的主要亚群的标志物。降钙素基因相关肽对胃总含量的贡献。
Retrograde tracing of the fluorescent marker. True Blue, has been used together with immunohistochemistry employing antibodies to substance P, calcitonin gene-related peptide, somatostatin, vasoactive intestinal polypeptide and morphine-modulating pcptide to study the afferent innervation of the stomach in rat, mouse and guinea-pig. Up to 85% of spinal afferents to the stomach in all three species contained immunoreactive calcitonin gene-related peptide. and up to 50% contained substance P. In all three species less than 10% of vagal afferents to the stomach reacted with antibodies to calcitonin generelated peptide. or substance P. Cacitonin gene-related pcptide-immunorcactive fibres were found in the myentcric plexus, circular muscle and around submucosal blood vessels in the stomach. In the rat, removal of the cocliac ganglion, splanchnic nerve section, or capsaicin treatment virtually abolished calcitonin gene-related peptide immunoreactivity in the stomach Capsaicin and splanchnie section also abolished the staining of immunoreactive calcitonin gene-related pcptide fibres in the coeliac ganglion. The same treatments abolished substance P staining of fibres around submucosal blood vessels, but in the myenteric plexus and circular smooth muscle there were still abundant immunoreactive fibres, presumably arising from intrinsic cell bodies. No somatostatin-containing visceral afferents could be found, although somatostatin was localized to cell bodies in rat dorsal root ganglia. Immunoreactive vasoactive intestinal polypeptide-containing dorsal root ganglia neurons were not found: although antibodies to morphinemodulalory pcptide revealed immunoreactive nerve cell bodies, we were unable to exclude the possibility that this result is attributable to cross reactivity with calcitonin gene-related peptide.These results provide direct evidence that calcitonin gene-related peptide is a marker for a major subset of visceral primary afferent neurons and suggest that this population of spinal afferents makes a major contribution to the total gastric content of calcitonin gene-related peptide.
降钙素基因相关肽：大鼠和狗胃酸分泌的有效外周抑制剂。
影响因子：
29.4
作者：
Tache,Y;Pappas,T;Lauffenburger,M;Goto,Y;Walsh,JH;Debas,H
通讯作者：
Debas,H
降钙素基因相关肽抑制酸分泌而不改变血流。
DOI：
10.1152/ajpgi.1987.252.2.g215
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Leung,FW;Tallos,EG;Tache,YF;Guth,PH
通讯作者：
Guth,PH
脑内注射降钙素基因相关肽抑制大鼠胃酸分泌。
影响因子：
6.1
作者：
Taché,Y;Gunion,M;Lauffenberger,M;Goto,Y
通讯作者：
Goto,Y