Altered gut microbial energy and metabolism in children with non-alcoholic fatty liver disease.
Altered gut microbial energy and metabolism in children with non-alcoholic fatty liver disease.
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DOI：
10.1093/femsec/fiu002
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发表时间：
2015-02
影响因子：
4.2
通讯作者：
Reo NV
中科院分区：
文献类型：
--
作者：
Michail S;Lin M;Frey MR;Fanter R;Paliy O;Hilbush B;Reo NV
肥胖症正在成为新的儿科流行病。非酒精性脂肪性肝病（NAFLD）通常与肥胖相关，并已成为儿童肝脏疾病的最常见原因。肠道微生物群是主要的代谢器官，决定了热量如何被处理，作为热量门，并有助于NAFLD的发病机制。本研究的目的是利用系统发育学、代谢组学、宏基因组学和蛋白质组学方法研究NAFLD儿童的肠道微生物特征。从患有或不患有NAFLD的肥胖儿童和健康的瘦弱儿童中获取粪便样本。粪便标本经16S rRNA基因芯片、霰弹枪测序、质谱分析蛋白质组学和核磁共振分析代谢物。NAFLD患儿的γ -变形菌和普雷沃氏菌含量更高，短链脂肪酸和乙醇含量也明显更高。该群体还增加了用于能量生产的基因组和蛋白质丰度，减少了碳水化合物和氨基酸代谢以及尿素循环和尿素运输系统。元蛋白质组和宏基因组显示相似的结果。儿童NAFLD的肠道微生物群不同于瘦弱的健康儿童，他们有更多的酒精产生和分配给能量代谢的途径，而不是碳水化合物和氨基酸代谢，这将有助于疾病的发展。
Obesity is becoming the new pediatric epidemic. Non-alcoholic fatty liver disease (NAFLD) is frequently associated with obesity, and has become the most common cause of pediatric liver disease. The gut microbiome is the major metabolic organ and determines how calories are processed, serving as a caloric gate, and contributing towards the pathogenesis of NAFLD. The goal of this study is to examine gut microbial profiles in children with NAFLD using phylogenetic, metabolomic, metagenomic, and proteomic approaches. Fecal samples were obtained from obese children with or without NAFLD and healthy lean children. Stool specimens were subjected to 16S rRNA gene microarray, shotgun sequencing, mass spectroscopy for proteomics, and NMR spectroscopy for metabolite analysis. Children with NAFLD had more abundant gamma-proteobacteria and Prevotella and significantly higher levels of short chain fatty acids, and ethanol. This group also had increased genomic and protein abundance for energy production with a reduction in carbohydrate and amino acid metabolism and urea cycle and urea transport systems. The metaproteome and metagenome showed similar findings. The gut microbiome in pediatric NAFLD is distinct from lean healthy children with more alcohol production and pathways allocated to energy metabolism over carbohydrate and amino acid metabolism, which would contribute to development of disease.
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DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
3.6
作者：
Maron, Pierre-Alain;Ranjard, Lionel;Lemanceau, Philippe
通讯作者：
Lemanceau, Philippe
影响因子：
120.7
作者：
Chiasson, JL;Josse, RG;Laakso, M
通讯作者：
Laakso, M
影响因子：
4.9
作者：
Michail, Sonia;Kenche, Harshavardhan
通讯作者：
Kenche, Harshavardhan
影响因子：
3.6
作者：
Anderson, Paul E.;Mahle, Deirdre A.;Raymer, Michael L.
通讯作者：
Raymer, Michael L.