Tissue tropism of the Musca domestica salivary gland hypertrophy virus.
Tissue tropism of the Musca domestica salivary gland hypertrophy virus.
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DOI：
10.1016/j.virusres.2010.06.015
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发表时间：
2011-01
期刊：
影响因子：
5
通讯作者：
Boucias, Drion G.
中科院分区：
文献类型：
--
作者：
Lietze, Verena-Ulrike;Salem, Tamer Z.;Prompiboon, Pannipa;Boucias, Drion G.
应用透射电镜和实时荧光定量PCR技术研究了家蝇唾液腺肥大病毒（MdSGHV）的组织嗜性。TEM表明，特征MdSGHV诱导的细胞核和细胞肥大仅限于唾液腺。唾液腺细胞中存在核衣壳和包膜病毒粒子。相反，中肠、卵巢、腹部脂肪体、农作物、气囊和脑的薄切片显示与这些组织相关的气管细胞空泡中存在包膜病毒粒子。然而，在气管细胞中没有检测到病毒形态发生的位点。MdSGHV DNA和转录物滴度的定量分析显示，病毒DNA存在于从MdSGHV感染的苍蝇收集的所有血淋巴和组织样品中。每50 ng DNA中的MdSGHV基因组拷贝数在检查的组织之间差异显著，范围从唾液腺样品中的3.83 × 108（± 3.75 × 107）到血淋巴样品中的7.98 × 105（± 2.91 × 105）。在中肠、脂肪体和脑样品中检测到高水平的病毒基因组拷贝。病毒转录本存在于所有检查的样品中，并且转录本丰度在唾液腺中也处于最高水平，在血淋巴中处于最低水平。然而，在分析的不同组织范围内，基因组拷贝的估计数量与病毒转录本之间没有相关性。病毒转录本在没有显示病毒形态发生位点的宿主组织中的功能仍有待阐明。
The tissue tropism of Musca domestica salivary gland hypertrophy virus (MdSGHV) infecting adult house flies was examined by transmission electron microscopy (TEM) and quantitative real-time PCR. TEM demonstrated that characteristic MdSGHV-induced nuclear and cellular hypertrophy was restricted to the salivary glands. Both nucleocapsids and enveloped virions were present in salivary gland cells. In contrast, thin sections of midguts, ovaries, abdominal fat body, crops, air sacs and brains showed the presence of enveloped virions in vacuoles of tracheal cells associated with these tissues. However, no sites of viral morphogenesis were detected in the tracheal cells. Quantitative analysis of MdSGHV DNA and transcript titers revealed that viral DNA was present in all hemolymph and tissue samples collected from MdSGHV-infected flies. Average numbers of MdSGHV genome copies per 50 ng of DNA varied significantly between examined tissues and ranged from 3.83 × 108 (± 3.75 × 107) in salivary gland samples to 7.98 × 105 (± 2.91 × 105) in hemolymph samples. High levels of viral genome copies were detected in midgut, fat body and brain samples. Viral transcripts were present in all examined samples, and transcript abundance was also at the highest level in salivary glands and at the lowest level in hemolymph. However, over the range of different tissues that were analyzed, there was no correlation between estimated quantities of genome copies and viral transcripts. The function of viral transcripts in host tissues that do not show sites of viral morphogenesis remains to be elucidated.
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影响因子：
5.4
作者：
MORRIS, TD;MILLER, LK
通讯作者：
MILLER, LK
DOI：
10.1073/pnas.91.8.3224
发表时间：
1994-04-12
影响因子：
11.1
作者：
ENGELHARD, EK;KAMMORGAN, LNW;VOLKMAN, LE
通讯作者：
VOLKMAN, LE
影响因子：
3.7
作者：
MORRIS, TD;MILLER, LK
通讯作者：
MILLER, LK
影响因子：
3.8
作者：
Salem, Tamer Z.;Garcia-Maruniak, Alejandra;Boucias, Drion G.
通讯作者：
Boucias, Drion G.
影响因子：
3.4
作者：
Lietze, Verena-U.;Sims, Kelly R.;Boucias, Drion G.
通讯作者：
Boucias, Drion G.