Scanning acoustic microscopy imaging of cellular structural and mechanical alterations from external stimuli.
Scanning acoustic microscopy imaging of cellular structural and mechanical alterations from external stimuli.
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DOI：
10.1016/j.heliyon.2021.e07847
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发表时间：
2021-08
期刊：
影响因子：
4
通讯作者：
Fukushi Y
中科院分区：
文献类型：
--
作者：
Miura K;Fukushi Y
外部刺激会对细胞造成结构和功能上的损伤。在扫描声学显微镜（SAM）下，声速（SOS）、衰减和厚度值被绘制出来，以显示细胞刚度、粘度和大小。然后使用统计分析对得到的数字数据进行比较。在本研究中，我们旨在利用SAM研究抗癌药物、酸性液体和微波负荷对癌细胞力学和结构特征的影响。我们发现，活性未经处理的细胞厚度增加，SOS和衰减减少，而死亡处理的细胞厚度减少，SOS增加。单宁、醋酸处理和微波辐照都增加了SOS和衰减，减少了厚度，这意味着这些处理使细胞更薄、更硬、更粘。此外，不同的抗癌药物与癌细胞相互作用，诱导SAM值的特征性变化。在光镜下很难观察到细胞的这些结构和力学变化。然而，在SAM作用下，癌细胞的活性和功能与SAM值的变化一致。对不同处理的细胞损伤参数进行统计学比较，建立细胞随时间的变化。因此，SAM观察可以可靠地评估化疗和物理治疗后癌细胞的损伤和恢复情况。这些结果可能有助于评价各种治疗方法的疗效。声学显微镜;声成像;声衰减;抗癌药物;癌症治疗;微波;细胞学;超声组织表征；丹宁酸;乙酸;病理
Cells incur structural and functional damage from external stimuli. Under scanning acoustic microscopy (SAM), speed of sound (SOS), attenuation, and thickness values are plotted to visualize cellular stiffness, viscosity, and size. The obtained digital data are then compared using statistical analysis. In the present study, we aimed to investigate the alterations in the mechanical and structural characteristics of cancer cells in response to anticancer drugs, acidic fluids, and microwave burdens using SAM. We found that active untreated cells showed increased thickness and reduced SOS and attenuation, whereas dying treated cells displayed reduced thickness and increased SOS. Tannic and acetic acid treatments and microwave irradiation all increased SOS and attenuation and reduced thickness, which meant that these treatments made cells thinner, stiffer, and more viscous. Furthermore, the different anticancer drugs interacted with cancer cells to induce characteristic changes in SAM values. These structural and mechanical alterations induced in cells were difficult to observe under light microscopy. However, under SAM, cancer cell activity and function corresponded consistently with changes in SAM values. Cellular damage parameters were statistically compared between the different treatments, and time-dependent cellular changes were established. SAM observation can therefore reliably evaluate cancer cell damage and recovery after chemotherapy and physical therapy. These results may help evaluate the therapeutic efficacy of various treatments. Acoustic microscopy; Acoustic imaging; Acoustic attenuation; Anticancer drugs; Cancer treatment; Microwave; Cytology; Ultrasonic tissue characterization; Tannic acid; Acetic acid; Pathology
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影响因子：
5.2
作者：
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