Tunica intima compensation for reduced stiffness of the tunica media in aging renal arteries as measured with scanning acoustic microscopy.
Tunica intima compensation for reduced stiffness of the tunica media in aging renal arteries as measured with scanning acoustic microscopy.
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DOI：
10.1371/journal.pone.0234759
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Miura K
中科院分区：
文献类型：
--
作者：
Miura K
衰老会导致肾动脉（RA）僵硬和功能下降。用光学显微镜进行组织学研究可以揭示微观结构重塑，但没有功能变化。本研究旨在通过使用扫描声学显微镜阐明 RA 结构和功能老化之间的关联。对 64 例尸检病例的福尔马林固定、石蜡包埋的肾动脉横截面进行了检查。比较不同年龄组之间对应于刚度的三层声速（SOS）值。根据血压 (BP) 和升主动脉的 SOS 检查中膜的 SOS。通过胶原酶处理后 SOS 的减少来评估对蛋白酶的脆弱性。内膜呈现向内肥厚且管腔变窄，中膜随着衰老呈现向外肥厚重塑。中膜和内外弹力层的SOS与年龄呈负相关。中膜的 SOS 与血压呈负相关，与主动脉的 SOS 呈强相关。与年老的RA相比，年轻的RA的中膜对胶原酶更敏感。扫描声学显微镜对于观察 RA 的老化过程非常有用。该技术同时显示 RA 每个部分的结构和机械信息。在衰老过程中，RA由于蛋白酶的消化而失去收缩功能和弹性。中膜和内外弹性层的硬度降低，但内膜因动脉粥样硬化而变硬。因此，RA的外形由圆形变为椭圆形，并向内和向外肥大。这表明内抵抗内膜支持中膜的机械弱点，以补偿随着年龄增长而增加的血压。
Aging causes stiffness and decreased function of the renal artery (RA). Histological study with light microscopy can reveal microscopic structural remodeling but no functional changes. The present study aimed to clarify the association between structural and functional aging of the RA through the use of scanning acoustic microscopy. Formalin-fixed, paraffin-embedded cross-sections of renal arteries from 64 autopsy cases were examined. Speed-of-sound (SOS) values of three layers, which correspond to the stiffness, were compared among different age groups. SOS of the tunica media was examined in terms of blood pressure (BP) and SOS of the ascending aorta. Vulnerability to proteases was assessed by SOS reduction after collagenase treatment. The tunica intima presented inward hypertrophy with luminal narrowing, and the tunica media showed outward hypertrophic remodeling with aging. SOS of the tunica media and internal and external elastic laminae showed a reverse correlation with age. SOS of the tunica media was negatively correlated with BP and strongly associated with that of the aorta. The tunica media of young RAs were more sensitive to collagenase compared with the old ones. Scanning acoustic microscopy is useful for observing the aging process of the RA. This technique simultaneously shows structural and mechanical information from each portion of the RA. In the process of aging, the RA loses contractile function and elasticity as a result of protease digestion. The tunica media and the internal and external elastic laminae exhibit reduced stiffness, but the tunica intima stiffens with atherosclerosis. As a consequence, the RA’s outer shape changes from round to oval with inward and outward hypertrophy. This indicates that the inner resistant intima supports the mechanical weakness of the tunica media to compensate for an increase in BP with aging.
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影响因子：
8.3
作者：
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通讯作者：
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通讯作者：
Sherratt, M. J.