Detection of Japanese encephalitis virus genotype V in Culex orientalis and Culex pipiens (Diptera: Culicidae) in Korea.
Detection of Japanese encephalitis virus genotype V in Culex orientalis and Culex pipiens (Diptera: Culicidae) in Korea.
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DOI：
10.1371/journal.pone.0116547
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Shin EH
中科院分区：
文献类型：
--
作者：
Kim H;Cha GW;Jeong YE;Lee WG;Chang KS;Roh JY;Yang SC;Park MY;Park C;Shin EH
日本脑炎病毒（JEV）引起严重的病毒性脑炎，分布在整个亚洲国家。据悉，该病毒是由主要在韩国稻田繁殖的三带喙库蚊传播的。在本研究中，我们调查了是否存在其他可以作为第二媒介或区域媒介传播乙脑病毒的蚊子。选取近5年发生乙脑病例的5个城市作为蚊虫采集点，根据蚊虫孳生地（牛棚、市区、森林、沼泽）将其细分为4个采集点。采用BG-Sentinel诱蚊器、CDC黑光诱蚊器、Fay-Prince诱蚊器和Gravid诱蚊器捕获蚊子。根据蚊虫种类、采集日期和采集地点，共编制蚊虫池993个，捕获蚊虫22774只。采用基于SYBR Green 1的实时RT-PCR检测蚊池中乙脑病毒。江原道和京畿道捕获的东方库蚊和淡色库蚊共检测到6个乙脑病毒阳性池。经包膜基因系统发育分析，所有检测到的流行性乙型流感病毒均为V型。我们的研究结果证实，韩国引入了一种新的乙脑病毒基因型，并提示两种蚊子可能在乙脑病毒传播中起作用。
Japanese encephalitis virus (JEV) causes significant viral encephalitis and is distributed throughout the Asian countries. The virus is known to be transmitted by Culex tritaeniorhynchus, which mainly breeds in rice paddies in Korea. In this study, we investigated the presence of other mosquito species that can transmit JEV as a second or regional vector. We selected five cities where patients have experienced JE in the last 5 years as mosquito-collecting locations and subdivided them into four collection sites according to the mosquito habitats (cowshed, downtown area, forest, and swamp). Mosquitoes were caught using the BG-Sentinel trap, CDC black-light trap, Fay-Prince trap, and Gravid trap. A total of 993 pools from 22,774 mosquitoes were prepared according to their species, collection date, and site. We performed a SYBR Green 1-based real-time RT-PCR assay to detect JEV from the mosquito pools. A total of six JEV-positive pools were detected from Culex orientalis and Culex pipiens caught in the Gangwon-do and Gyeonngi-do provinces. All the detected JEVs were revealed as genotype V by phylogenetic analysis of the envelope gene. Our findings confirm that a new genotype of JEV was introduced in Korea and suggest that two mosquito species may play a role in JEV transmission.
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影响因子：
3.8
作者：
Li MH;Fu SH;Chen WX;Wang HY;Guo YH;Liu QY;Li YX;Luo HM;Da W;Duo Ji DZ;Ye XM;Liang GD
通讯作者：
Liang GD
影响因子：
4.8
作者：
Takhampunya R;Kim HC;Tippayachai B;Kengluecha A;Klein TA;Lee WJ;Grieco J;Evans BP
通讯作者：
Evans BP
影响因子：
4.8
作者：
Yun, Seok-Min;Cho, Jung Eun;Jeong, Young Eui
通讯作者：
Jeong, Young Eui
影响因子：
3.1
作者：
Yang, Cheng-Fen;Chen, Chien-Fu;Wu, Ho-Sheng
通讯作者：
Wu, Ho-Sheng
影响因子：
2.1
作者：
Balenghien, T.;Fouque, F.;Bicout, D. J.
通讯作者：
Bicout, D. J.