Genotype v Japanese encephalitis virus is emerging.
Genotype v Japanese encephalitis virus is emerging.
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DOI：
10.1371/journal.pntd.0001231
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发表时间：
2011-07
影响因子：
3.8
通讯作者：
Liang GD
中科院分区：
文献类型：
--
作者：
Li MH;Fu SH;Chen WX;Wang HY;Guo YH;Liu QY;Li YX;Luo HM;Da W;Duo Ji DZ;Ye XM;Liang GD
日本脑炎(JE)是一种全球性公共卫生问题，已广泛传播到亚洲20多个国家，并将地理范围扩大到包括澳大利亚在内的南太平洋地区。乙脑已成为世界上病毒性脑炎的最重要原因。根据日本脑炎病毒(JEV)外膜蛋白(E)基因的核苷酸序列，将其分为5种基因类型。Muar株是1952年从马来亚患者身上分离出来的，是V型JEV的唯一例子。在此，从中国采集的三带喙库蚊中分离到乙脑病毒XZ0934株。测定了XZ0934株的核苷酸和氨基酸全序列。XZ0934与已知的I-IV型JEV分离株的核苷酸差异为20.3%~21.4%，氨基酸差异为8.4%~10.0%，表明XZ0934与I-IV型JEVS的相似性较低。用全基因组和结构基因核苷酸序列进行的系统发育分析表明，XZ0934属于V型，这表明V型JEV正在乙脑流行地区出现。因此，加强对V型乙脑病毒引起的病毒性脑炎的监测和诊断是乙脑病毒流行国家非常关注的问题。乙型脑炎病毒(JEV)是一种由蚊媒传播的病毒，可引起乙脑，具有显著的发病率和死亡率。根据乙型脑炎病毒(JEV)外膜蛋白(E)基因的核苷酸序列，已鉴定出5种基因类型(I-V型)。到目前为止，唯一已知的V型毒株是1952年从马来亚患者身上分离的Muar毒株。自那以后，世界上没有检测到V型乙脑病毒。本研究从2009年中国采集的蚊虫标本中分离到乙脑病毒XZ0934株。通过全基因组和结构基因序列的系统发育分析，确定XZ0934株为V型乙脑病毒第二株。这表明V型乙脑病毒在57年后(1952-2009年)再次出现。因此，需要加强对V型乙脑病毒引起的乙脑病例的监测和更有效的诊断。
Japanese encephalitis (JE) is a global public health issue that has spread widely to more than 20 countries in Asia and has extended its geographic range to the south Pacific region including Australia. JE has become the most important cause of viral encephalitis in the world. Japanese encephalitis viruses (JEV) are divided into five genotypes, based on the nucleotide sequence of the envelope (E) gene. The Muar strain, isolated from patient in Malaya in 1952, is the sole example of genotype V JEV. Here, the XZ0934 strain of JEV was isolated from Culex tritaeniorhynchus, collected in China. The complete nucleotide and amino acid sequence of XZ0934 strain have been determined. The nucleotide divergence ranged from 20.3% to 21.4% and amino acid divergence ranged from 8.4% to 10.0% when compared with the 62 known JEV isolates that belong to genotype I–IV. It reveals low similarity between XZ0934 and genotype I–IV JEVs. Phylogenetic analysis using both complete genome and structural gene nucleotide sequences demonstrates that XZ0934 belongs to genotype V. This, in turn, suggests that genotype V JEV is emerging in JEV endemic areas. Thus, increased surveillance and diagnosis of viral encephalitis caused by genotype V JEV is an issue of great concern to nations in which JEV is endemic. Japanese encephalitis virus (JEV) is a mosquito-borne virus that causes Japanese encephalitis (JE) with significant morbidity and mortality. Five genotypes (genotype I–V) have been identified based on the nucleotide sequence of viral envelope (E) gene of JEV. To date, the only known strain of genotype V is Muar strain, isolated from patient in Malaya in 1952. Since then, no genotype V JEV has been detected in the world. In this study, the JEV strain, XZ0934, was isolated from mosquito samples collected in China in 2009. The full-length genome sequences of the XZ0934 strain was determined and founded to be the second strain of genotype V JEV based on the phylogenetic analysis using the complete genome and structural gene sequences. This suggests that genotype V JEV is re-emerging after 57 years (1952–2009). Therefore, increased surveillance and more effective diagnosis for cases of JE caused by genotype V JEV are needed.
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