Molecular and Bioinformatics Analyses Identify 7 Circular RNAs Involved in Regulation of Oncogenic Transformation and Cell Proliferation in Human Bladder Cancer.
Molecular and Bioinformatics Analyses Identify 7 Circular RNAs Involved in Regulation of Oncogenic Transformation and Cell Proliferation in Human Bladder Cancer.
复制标题
DOI：
10.12659/msm.908837
复制
发表时间：
2018-03-20
期刊：
影响因子：
--
通讯作者：
Kong G
中科院分区：
文献类型：
--
作者：
Cai D;Liu Z;Kong G
环状RNAs(CircRNAs)已成为肿瘤发生和转移的重要调节因子。然而，对CircRNAs在膀胱癌中的作用的了解仍然有限。本研究旨在探讨CircRNAs在膀胱癌发生发展中的作用及机制。收集患者膀胱癌(包括高、低级别肿瘤)及癌旁正常组织3对。提取总RNA，经Clariom D芯片分析检测差异表达的CircRNAs和mRNAs。Miranda结合严格的差异mRNA过滤器预测了这些CircRNAs的mRNA靶标。CircRNA-mRNA对的相互作用网络由Cytoscape生成。在Clariom D芯片检测到的1038个CircRNA中，我们发现了7个在肿瘤中显著差异表达的CircRNA(折叠式变化2，FDR<；0.05)。对差异CircRNA的主成分分析证实，肿瘤标本与正常标本是分离的。对这些RNA及其预测的mRNA靶点进行的系统聚类分析表明，大多数差异表达的CircRNAs和mRNAs在膀胱肿瘤中表达上调。KEGG信号通路分析表明，参与细胞增殖、致癌转化和转移的基因可能受这些CircRNAs的调控。本研究为进一步研究CircRNAs调控膀胱癌的机制提供了分子基础。已鉴定的CircRNAs作为膀胱癌患者的诊断和预后生物标记物的潜力突出了其临床意义。
Circular RNAs (circRNAs) have emerged as important regulators in carcinogenesis and metastasis. However, the knowledge of circRNAs in bladder cancer remains limited. This study aimed to investigate the role and mechanism of circRNAs in the development and progression of bladder cancer. Three pairs of bladder carcinomas (including high- and low-grade tumors) and adjacent normal tissues were collected from patients. The total RNAs were extracted from these samples and subjected to Clariom D microarray assays to detect the differentially expressed circRNAs and mRNAs. The mRNA targets for these circRNAs were predicted by miRanda in combination with stringent differential mRNA filters. The interaction network for the circRNA-mRNA pairs was generated by Cytoscape. Among the 1038 circRNAs detected by the Clariom D microarray assay, we identified 7 significantly differentially expressed circRNAs in the tumors (fold change >2, FDR <0.05). Principal component analysis of the differential circRNAs confirmed that the tumor samples were separated from the normal samples. Hierarchical clustering analyses on these RNAs and their predicted mRNA targets showed that the majority of differentially expressed circRNAs and mRNAs had been up-regulated in the bladder tumors. KEGG signaling pathway analysis has indicated that genes involved in cell proliferation, oncogenic transformation, and metastasis are potentially regulated by these circRNAs. The current study provides a molecular basis for further investigating the mechanisms by which circRNAs regulate bladder cancer. The clinical significance of the identified circRNAs is highlighted by their potentials as diagnostic and prognostic biomarkers for bladder cancer patients.
登录
查看更多内容
影响因子：
4.6
作者：
Bachmayr-Heyda A;Reiner AT;Auer K;Sukhbaatar N;Aust S;Bachleitner-Hofmann T;Mesteri I;Grunt TW;Zeillinger R;Pils D
通讯作者：
Pils D
影响因子：
11.5
作者：
Platt, Fiona M.;Hurst, Carolyn D.;Knowles, Margaret A.
通讯作者：
Knowles, Margaret A.
影响因子：
254.7
作者：
Siegel, Rebecca;Ma, Jiemin;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin
影响因子：
28.5
作者：
Dong Y;He D;Peng Z;Peng W;Shi W;Wang J;Li B;Zhang C;Duan C
通讯作者：
Duan C
DOI：
10.1158/1541-7786.mcr-09-0329
发表时间：
2010-08
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Zhong M;Boseman ML;Millena AC;Khan SA
通讯作者：
Khan SA