Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan
Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan
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整个脊椎动物生命周期中肌源性前体细胞的克隆行为
DOI：
10.1101/2022.02.17.480906
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hughes S
中科院分区：
文献类型：
--
作者：
Hughes S
为了解决骨骼肌发生过程中干细胞多样性的问题，一种类似Brainbow的遗传细胞谱系追踪方法，命名为Musclebow 2，是通过增强子捕获在斑马鱼中得到的。结果表明，在初级肌节初步形成后，至少有15个肌肉前体细胞（mpcs）种子每个体节，在那里他们增殖，但有助于肌肉生长孵化前。此后，皮肌节来源的mpc克隆迅速扩大，而一些后代进行终末分化，导致随机克隆漂移的mpc池。没有证据表明基于细胞谱系的克隆命运多样性。在未受伤的动物中未观察到纤维或mpc死亡。个体标记的肌纤维在大部分寿命中持续存在，表明核周转率低。在成年期，早期标记的mpc克隆标记稳定的组织块，包括轴上或轴下体节的重要部分。来自单独的早期标记的克隆的细胞融合发生在克隆重叠的区域中。伤口从几个局部mpc再生;没有获得专门的干mpc的证据。总之，我们的数据表明，肌肉组织中的大多数mpc有助于局部生长和修复，并表明在没有创伤的情况下，细胞周转率很低。
To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbedMusclebow2, was derived by enhancer trapping in zebrafish. It is shown that, after initial formation of the primary myotome, at least 15 muscle precursor cells (mpcs) seed each somite, where they proliferate but contribute little to muscle growth prior to hatching. Thereafter, dermomyotome-derived mpc clones rapidly expand while some progeny undergo terminal differentiation, leading to stochastic clonal drift within the mpc pool. No evidence of cell-lineage-based clonal fate diversity was obtained. Neither fibre nor mpc death was observed in uninjured animals. Individual marked muscle fibres persist across much of the lifespan indicating low rates of nuclear turnover. In adulthood, early-marked mpc clones label stable blocks of tissue comprising a significant fraction of either epaxial or hypaxial somite. Fusion of cells from separate early-marked clones occurs in regions of clone overlap. Wounds are regenerated from several local mpcs; no evidence for specialised stem mpcs was obtained. In conclusion, our data indicate that most mpcs in muscle tissue contribute to local growth and repair and suggest that cellular turnover is low in the absence of trauma.
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影响因子：
23.9
作者：
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影响因子：
2.5
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通讯作者：
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影响因子：
2.7
作者：
Hirsinger, E;Stellabotte, F;Westerfield, M
通讯作者：
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