Zebrafish jam‐b2 Gal4‐enhancer trap line recapitulates endogenous jam‐b2 expression in extraocular muscles
Zebrafish jam‐b2 Gal4‐enhancer trap line recapitulates endogenous jam‐b2 expression in extraocular muscles
复制标题
斑马鱼 jamâb2 Gal4âenhancer trap line 重现眼外肌中内源性 jamâb2 表达
DOI：
10.1002/dvdy.24347
复制
发表时间：
2015
影响因子：
2.5
通讯作者：
R. W. Köster
中科院分区：
文献类型：
--
作者：
H. Matsui;A. Dorigo;A. Buchberger;J. C. Hocking;M. Distel;R. W. Köster
背景连接粘附分子（JAM）家族成员作为细胞粘附分子和细胞表面受体发挥作用。斑马鱼基因组包含六种不同的jam基因，jam ‐bandjam‐ c被证明是骨骼肌发育过程中成肌细胞融合所必需的。然而，鲜为人知的是aboutjam-b2的表达和function.ResultsWe分离的cDNA的zebrafishjam-b2. jam-b2是专门表达在眼外肌（EOMs），颌骨肌肉，和胸鳍在斑马鱼幼虫，但不是在躯干肌肉。对斑马鱼Gal 4增强子捕获系的分析支持了所鉴定的jam-b2表达模式，其中将转录激活因子KalTA 4的编码序列与Gal 4依赖性UAS-mCherry表达盒一起插入jam-b2位点。与UAS：EGFP菌株的杂交证明了将转基因表达靶向至EOM、颌肌和鳍的可能性。最后，我们的特点是一致的收缩模式的眼外肌在幼虫执行optokinetic response.ConclusionsThe表达模式ofjam-b2表明，它可能有助于不同的属性，眼外肌，颌肌，和胸鳍。Thejam-b2：KalTA 4-UAS-mCherry转基因菌株作为这些肌肉的报告基因和作为将转基因表达靶向EOMs的有价值的遗传工具发挥双重作用。Developmental Dynamics 244：1574-1580，2015。© 2015 Wiley Periodicals，Inc.
BackgroundMembers of the junctional adhesion molecule (JAM) family function as cell adhesion molecules and cell surface receptors. The zebrafish genome contains six differentjamgenes, andjam‐bandjam‐cwere shown to be essential for myoblast fusion during skeletal muscle development. However, little is known aboutjam‐b2expression and function.ResultsWe isolated the cDNA of zebrafishjam‐b2.jam‐b2is expressed specifically in extraocular muscles (EOMs), jaw muscles, and pectoral fins in zebrafish larvae, but not in trunk muscles. The identifiedjam‐b2expression pattern is supported by the analysis of a zebrafish Gal4‐enhancer trap line, in which the coding sequence of the transcriptional activator KalTA4 together with a Gal4‐dependent UAS‐mCherry expression cassette was inserted into thejam‐b2locus. Intercrosses with an UAS:EGFP strain proves the possibility for targeting transgene expression to EOMs, jaw muscles and fins. Finally, we characterized the concerted contraction pattern of EOMs in larvae performing an optokinetic response.ConclusionsThe expression pattern ofjam‐b2suggests that it may contribute different properties to EOMs, jaw muscles, and pectoral fins. Thejam‐b2:KalTA4‐UAS‐mCherry transgenic strain serves a dual role as both a reporter for these muscles and as a valuable genetic tool for targeting transgene expression to EOMs.Developmental Dynamics 244:1574–1580, 2015. © 2015 Wiley Periodicals, Inc.
登录
查看更多内容
影响因子：
6.4
作者：
H. Mühlendyck
通讯作者：
H. Mühlendyck
DOI：
10.1073/pnas.1403105111
发表时间：
2014-08-12
影响因子：
11.1
作者：
Matsui, Hideaki;Namikawa, Kazuhiko;Koester, Reinhard W.
通讯作者：
Koester, Reinhard W.
影响因子：
3.5
作者：
Kelly, RG;Jerome-Majewska, LA;Papaioannou, VE
通讯作者：
Papaioannou, VE
影响因子：
2.8
作者：
Yu, CY;Man, W;Griffiths, PG
通讯作者：
Griffiths, PG
影响因子：
2.6
作者：
Kuratani S
通讯作者：
Kuratani S