Parenteral and oral immunization with a plasmid DNA expressing the human papillomavirus 16‐L1 gene induces systemic and mucosal antibodies and cytotoxic T lymphocyte responses
Parenteral and oral immunization with a plasmid DNA expressing the human papillomavirus 16‐L1 gene induces systemic and mucosal antibodies and cytotoxic T lymphocyte responses
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使用表达人乳头瘤病毒 16-L1 基因的质粒 DNA 进行肠胃外和口服免疫可诱导全身和粘膜抗体以及细胞毒性 T 淋巴细胞反应
DOI：
10.1002/jmv.2115
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发表时间：
2002
影响因子：
12.7
通讯作者：
A. García
中科院分区：
文献类型：
--
作者：
L. Rocha;Jorge E. Alejandre;A. García
人乳头瘤病毒（HPV）感染与宫颈癌的关系已被证实。因此，开发预防性疫苗来预防原发性HPV感染可能是降低全球这种癌症发病率的有效手段。为了评估表达HPV 16型L1基因的质粒DNA诱导保护性免疫应答的能力，通过胃肠外和口服途径免疫小鼠。肌肉注射、皮下注射和口服DNA疫苗的动物产生了全身性抗L1 IgG抗体。在皮下接种的小鼠中产生的抗体在免疫后12个月可检测到。在免疫小鼠的阴道洗液中也发现了特异性伊加抗体。在替代中和试验中，全身和局部抗体均被证明有效。从实验小鼠中提取的脾T细胞显示由CD 8+细胞介导的细胞毒性T淋巴细胞（CTL）活性。用经改造以表达HPV 16-L1蛋白的同基因黑色素瘤细胞系对小鼠进行攻击，接种疫苗的动物中的肿瘤显示出较慢的生长速度，这与小鼠的更长生存期直接相关。结果表明，基于L1的DNA疫苗可能有助于预防HPV 16的原发感染。医学病毒学杂志66：86-95，2002.© 2002 Wiley利斯公司
The association of human papillomavirus (HPV) infection and cervical cancer has been demonstrated. The development of a prophylactic vaccine to protect against primary HPV infection may therefore be an efficient means to reduce the incidence of this cancer worldwide. To assess the capacity of a plasmid DNA that expresses the L1 gene of HPV type 16 to induce a protective immune response, mice were immunized by parenteral and oral routes. Animals that received the DNA vaccine intramuscularly, subcutaneously and orally, developed systemic anti‐L1 IgG antibodies. Antibodies developed in mice vaccinated subcutaneously were detectable twelve months post‐immunization. Specific IgA antibodies were also found in vaginal washes from immunized mice. Both systemic and local antibodies proved effective in a surrogate neutralization assay. Splenic T cells extracted from experimental mice showed cytotoxic T lymphocytes (CTL) activity mediated by CD8 + cells. Mice were challenged with a syngeneic melanoma cell line, engineered to express the HPV16‐L1 protein, tumours in vaccinated animals showed slower growth rate, correlated directly with a longer survival of mice. The results suggest that the L1‐based DNA vaccine may be useful for the prevention of primary infections by HPV16. J. Med. Virol. 66:86–95, 2002. © 2002 Wiley‐Liss, Inc.
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影响因子：
3.5
作者：
Wolff, Jon A.;Ludtke, James J.;Jani, Agnes
通讯作者：
Jani, Agnes
颗粒介导的重组表达载体递送至兔皮肤可诱导高滴度的多克隆抗血清（并避免蛋白质免疫原的纯化）。
影响因子：
14.9
作者：
Sundaram,P;Xiao,W;Brandsma,JL
通讯作者：
Brandsma,JL
DOI：
10.1073/pnas.95.4.1800
发表时间：
1998-02-17
影响因子：
11.1
作者：
Greenstone, HL;Nieland, JD;Schiller, JT
通讯作者：
Schiller, JT
给兔子皮内接种棉尾兔乳头瘤病毒 (CRPV) L1 基因疫苗可以防止病毒攻击。
影响因子：
5.5
作者：
Sundaram,P;Tigelaar,RE;Brandsma,JL
通讯作者：
Brandsma,JL
DNA 直接转移至骨骼肌后对 MHC I 类抗原产生免疫。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Geissler,EK;Wang,J;FechnerJr,JH;Burlingham,WJ;Knechtle,SJ
通讯作者：
Knechtle,SJ