Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus
Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus
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摩尔达维青兰提取物对金黄色葡萄球菌临床分离株的抗菌活性及作用机制
DOI：
10.3389/fmicb.2019.01249
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发表时间：
2019-06
影响因子：
5.2
通讯作者：
Wang Zhanli
中科院分区：
文献类型：
--
作者：
Yu Hui;Liu Min;Liu Yun;Qin Lei;Jin Min;Wang Zhanli
背景：香青兰是世界上许多国家普遍使用的传统药物，具有广泛的药理作用。本研究的目的是研究D. [医]莫达维卡湖提取物对金黄色葡萄球菌的临床分离株。我们的结果表明，50%和90%的S.金黄色葡萄球菌分离物的乙酸乙酯（EtOAc）级分的MIC 50和MIC 90。[医]莫达维卡湖乙醇提取物分别为780和1,065 μg/ml。我们进一步观察到破坏24小时生物膜的EtOAc级分引起细胞膜损伤和不规则的细胞形状。此外，EtOAc级分对人表皮角质形成细胞（HaCaT）细胞显示出轻微至中度的毒性作用。差异蛋白质组分析结果显示，在S.在用EtOAc级分处理后的金黄色葡萄球菌中。通过基因本体论（GO）富集和京都基因和基因组百科全书（KEGG）途径对差异表达的蛋白质进行功能分类。这些蛋白质有助于膜损伤、生物膜形成的抑制和能量代谢的变化。因此，D. [医]莫达维卡湖乙醇提取物作为一种天然产物，具有作为抗菌剂控制临床分离的沙门氏菌的潜力。金黄色。
Background: Dracocephalum moldavica L. is a popular traditional medicine used by many countries, which has a wide range of pharmacological effects. The aim of this work was to investigate the antimicrobial effects of D. moldavica L. extracts against clinical isolates of Staphylococcus aureus. Our results demonstrated that the minimal inhibitory concentration (MIC) for 50 and 90% of S. aureus isolates (MIC50 and MIC90) of the ethyl acetate (EtOAc) fraction from D. moldavica L. ethanol extract were 780 and 1,065 μg/ml, respectively. We further observed that the EtOAc fraction disrupted 24-h biofilm caused cell membrane damage and irregular cell shape. Additionally, the EtOAc fraction showed slight to moderate toxic effects on human epidermal keratinocyte (HaCaT) cells. Moreover, the results of the differential proteome revealed that 231 proteins were upregulated, while 61 proteins were downregulated in S. aureus after treatment with the EtOAc fraction. The differentially expressed proteins were functionally categorized by the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins contribute to membrane damage, inhibition of biofilm formation, and changes in energy metabolism. Thus, the EtOAc fraction of D. moldavica L. ethanol extract, as a natural product, has the potential to be used as an antimicrobial agent to control the clinical isolates of S. aureus.
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影响因子：
3
作者：
Wendl MC
通讯作者：
Wendl MC
影响因子：
3.7
作者：
Garcia SS;Du Q;Wu H
通讯作者：
Wu H
影响因子：
2.2
作者：
Jun-Liang Zhang;Ren-jie Yan;Ning Yu;Xue Zhang;Dai-Jie Chen;Tong Wu;Jianguo Xin
通讯作者：
Jun-Liang Zhang;Ren-jie Yan;Ning Yu;Xue Zhang;Dai-Jie Chen;Tong Wu;Jianguo Xin
DOI：
10.5455/jice.20160906035020
发表时间：
2016-01-01
期刊：
JOURNAL OF INTERCULTURAL ETHNOPHARMACOLOGY
影响因子：
--
作者：
Ekambaram, Sanmuga Priya;Perumal, Senthamil Selvan;Viswanathan, Vinodhini
通讯作者：
Viswanathan, Vinodhini
影响因子：
11.4
作者：
M. McDonald;A. Hurse;Kwang N. Sim
通讯作者：
M. McDonald;A. Hurse;Kwang N. Sim