Origin and Characterization of Extracellular Vesicles Present in the Spider Venom of Ornithoctonus hainana.
Origin and Characterization of Extracellular Vesicles Present in the Spider Venom of Ornithoctonus hainana.
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海南鸟蛛蜘蛛毒液中细胞外囊泡的起源和特征
DOI：
10.3390/toxins13080579
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发表时间：
2021-08-20
期刊：
影响因子：
4.2
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Xun C;Wang L;Yang H;Xiao Z;Deng M;Xu R;Zhou X;Chen P;Liu Z
细胞外囊泡（EV），包括外泌体和微泡，是几乎所有细胞类型释放的膜囊泡。它们含有各种生物活性分子，其分子组成因其细胞来源而异。随着对有毒动物研究的进展，在蛇和寄生黄蜂的毒液中发现了 EV。尽管已经观察到蜘蛛毒腺中的囊泡分泌，但这些分泌囊泡的起源和生物学特性尚不清楚。在这项研究中，使用透射电子显微镜（TEM）观察了海南鸟毒液中 EV 的起源。通过密度梯度离心分离纯化海南鸟蛇毒液胞外囊泡（HN-EV）。 HN-EV 具有经典的膜囊泡，尺寸分布范围为 50 至 150 nm，并表达节肢动物 EV 标记 Tsp29Fb。 LC-MS/MS 分析共鉴定出 150 个蛋白质，根据其潜在功能分为三组：保守性囊泡转运相关蛋白、毒力相关蛋白和其他功能未知的蛋白质。从功能上讲，HN-EV具有透明质酸酶活性，通过影响细胞骨架和细胞周期来抑制人脐静脉内皮细胞（HUVEC）的增殖。总的来说，这项研究首次研究了 HN-EV 的生物学特性，并为蜘蛛毒液的中毒过程提供了新的线索。
Extracellular vesicles (EVs), including exosomes and microvesicles, are membranous vesicles released from nearly all cellular types. They contain various bioactive molecules, and their molecular composition varies depending on their cellular origin. As research into venomous animals has progressed, EVs have been discovered in the venom of snakes and parasitic wasps. Although vesicle secretion in spider venom glands has been observed, these secretory vesicles’ origin and biological properties are unknown. In this study, the origin of the EVs from Ornithoctonus hainana venom was observed using transmission electron microscopy (TEM). The Ornithoctonus hainana venom extracellular vesicles (HN-EVs) were isolated and purified by density gradient centrifugation. HN-EVs possess classic membranous vesicles with a size distribution ranging from 50 to 150 nm and express the arthropod EV marker Tsp29Fb. The LC-MS/MS analysis identified a total of 150 proteins, which were divided into three groups according to their potential function: conservative vesicle transport-related proteins, virulence-related proteins, and other proteins of unknown function. Functionally, HN-EVs have hyaluronidase activity and inhibit the proliferation of human umbilical vein endothelial cells (HUVECs) by affecting the cytoskeleton and cell cycle. Overall, this study investigates the biological characteristics of HN-EVs for the first time and sheds new light on the envenomation process of spider venom.
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DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
3.6
作者：
Klint, Julie K.;Chin, Yanni K. -Y.;Mobli, Mehdi
通讯作者：
Mobli, Mehdi
影响因子：
3.7
作者：
Biner O;Trachsel C;Moser A;Kopp L;Langenegger N;Kämpfer U;von Ballmoos C;Nentwig W;Schürch S;Schaller J;Kuhn-Nentwig L
通讯作者：
Kuhn-Nentwig L
影响因子：
--
作者：
Khamtorn P;Rungsa P;Jangpromma N;Klaynongsruang S;Daduang J;Tessiri T;Daduang S
通讯作者：
Daduang S
影响因子：
4.2
作者：
Kuhn-Nentwig, Lucia;Langenegger, Nicolas;Nentwig, Wolfgang
通讯作者：
Nentwig, Wolfgang