Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines.
Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines.
复制标题
中药炮制抗流感活性物质的筛选
DOI：
10.1155/2020/4979850
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Du R
中科院分区：
文献类型：
--
作者：
Kang Q;Wang Y;Cui Q;Gong L;Yang Y;Jiang H;Rong L;Rong R;Du R
Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50's of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic.
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DOI：
10.3390/molecules13061406
发表时间：
2008-06-20
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Wagenaar MM
通讯作者：
Wagenaar MM
DOI：
10.1021/ci0200467
发表时间：
2003-01-01
期刊：
JOURNAL OF CHEMICAL INFORMATION AND COMPUTER SCIENCES
影响因子：
--
作者：
Feher, M;Schmidt, JM
通讯作者：
Schmidt, JM
影响因子：
4.6
作者：
Fu YF;Jiang LH;Zhao WD;Xi-Nan M;Huang SQ;Yang J;Hu TJ;Chen HL
通讯作者：
Chen HL
影响因子：
168.9
作者：
Bright, RA;Medina, MJ;Klimov, AI
通讯作者：
Klimov, AI
影响因子：
2.8
作者：
Fatima, Munazza;Zaidi, Najam-us-Sahar Sadaf;Afzal, Farhan
通讯作者：
Afzal, Farhan