Essential genes and deficiencies in the unc-22 IV region of Caenorhabditis elegans.
Essential genes and deficiencies in the unc-22 IV region of Caenorhabditis elegans.
复制标题
秀丽隐杆线虫 unc-22 IV 区域的必需基因和缺陷。
作者：
T. Rogalski;D. Moerman;D. Baillie
分离并定位了五种由甲醛引起的缺陷，这些缺陷揭示了 unc-22 IV（一种影响线虫肌肉结构的基因）。最大的缺陷 sDf2 从 unc-22 开始向两个方向延伸，长度约为 1.0-2.0 个地图单位。其他四个缺陷 sDf7、sDf8、sDf9 和 sDf10 均小于 sDf2，且位于该缺陷未覆盖的区域内。分离了 37 个与 unc-22 相关的甲磺酸乙酯诱导的致死和不育突变，并测试了与 sDf2 的互补性。 19 个致命突变未能补充 sDf2。其中 16 个通过重组作图以及 sDf7、sDf8、sDf9 和 sDf10 的缺陷作图进一步定位。这 16 个突变定义了该区域的 11 个新的必需基因。其中八个基因位于 unc-22 左侧 0.9 个图谱单位间隔内，而其余三个基因位于 unc-22 右侧约 0.2 个图谱单位的区域内。我们认为，本研究中确定的两个必需基因 let-56 和 let-52 是 unc-22 两侧的相邻基因。致死突变表现出多种终末表型：从第一阶段幼虫到不育成虫。
Five formaldehyde-induced deficiencies that uncover unc-22 IV, a gene affecting muscle structure in the nematode Caenorhabditis elegans were isolated and positioned. The largest deficiency, sDf2, extends in both directions from unc-22 and is approximately 1.0-2.0 map units in length. The other four deficiencies, sDf7, sDf8, sDf9 and sDf10, are all smaller than sDf2 and are located within the region uncovered by this deficiency. Thirty-seven ethyl methane-sulfonate-induced lethal and sterile mutations linked to unc-22 were isolated and tested for complementation with sDf2. Nineteen lethal mutations failed to complement sDf2. Sixteen of these were further positioned by recombination mapping and also by deficiency mapping with sDf7, sDf8, sDf9 and sDf10. These sixteen mutations define 11 new essential genes in this region. Eight of the genes lie in a 0.9-map unit interval to the left of unc-22, whereas the three remaining genes lie in a region of about 0.2 map units to the right of unc-22. We believe that two of the essential genes identified in this study, let-56 and let-52, are the adjacent genes on either side of unc-22. The lethal mutations exhibit a wide range of terminal phenotypes: from first stage larva to sterile adult.
秀丽隐杆线虫 X 连锁致死和不育突变的抑制和功能。
影响因子：
3.3
作者：
Meneely,PM;Herman,RK
通讯作者：
Herman,RK
秀丽隐杆线虫中的第二个信息抑制因子 SUP-7 X。
影响因子：
3.3
作者：
Waterston,RH
通讯作者：
Waterston,RH