Cell cycle-related and expression-elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non-small-cell lung cancer.
Cell cycle-related and expression-elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non-small-cell lung cancer.
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DOI：
10.1111/cas.13524
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发表时间：
2018-04
期刊：
影响因子：
5.7
通讯作者：
Jin F
中科院分区：
文献类型：
--
作者：
Li W;Zheng G;Xia J;Yang G;Sun J;Wang X;Wen M;Sun Y;Zhang Z;Jin F
肿瘤中细胞周期相关和表达升高的蛋白 (CREPT) 在多种人类恶性肿瘤中过度表达。然而，CREPT 表达的临床相关性及其在非小细胞肺癌 (NSCLC) 中的生物学作用仍不清楚。在本研究中，我们通过免疫组织化学、Western blot 分析和 RT-PCR 检测了 NSCLC 组织和细胞系中 CREPT 的表达。在 271 例 NSCLC 患者中分析了 CREPT 表达与临床病理特征之间的相关性。通过Kaplan-Meier分析和Cox回归分析评估CREPT表达的预后价值。 使用质粒载体在 Calu-1 细胞系中过表达 CREPT，并在体外和体内探索其生物学功能。我们发现，与配对的邻近非肿瘤组织相比，CREPT在NSCLC中显着过表达，并且CREPT的表达水平与肿瘤分化、淋巴结转移和临床分期相关。 Kaplan-Meier分析显示，CREPT高表达组的无复发生存期和总生存期显着短于CREPT低表达组。多变量分析表明，CREPT 可能是预测 NSCLC 预后的独立生物标志物。 CREPT 的过度表达会增加细胞增殖，并增强 Calu-1 细胞（一种 CRPET 表达相对较低的人类 NSCLC 细胞系）的体外迁移和侵袭能力。此外，CREPT 过表达促进了裸鼠模型中的肿瘤生长。这些结果表明CREPT与NSCLC细胞的增殖密切相关，可能是NSCLC患者潜在的预后标志物。
The cell cycle‐related and expression‐elevated protein in tumor (CREPT) is overexpressed in several human malignancies. However, the clinical relevance of CREPT expression and its biological role in non‐small‐cell lung cancer (NSCLC) remains unclear. In this study, we detected the expression of CREPT in both NSCLC tissues and cell lines by immunohistochemistry, Western blot analysis, and RT‐PCR. The correlation between CREPT expression and clinicopathologic features was analyzed in 271 NSCLC patients. The prognostic value of CREPT expression was evaluated by Kaplan–Meier analysis and Cox regression analysis. CREPT was overexpressed in Calu‐1 cell lines by using plasmid vector and its biological function was explored both in vitro and in vivo. We found that CREPT was significantly overexpressed in NSCLC compared with paired adjacent non‐tumor tissues, and the expression level of CREPT was correlated with tumor differentiation, lymph node metastasis, and clinical stage. Kaplan–Meier analysis showed that the recurrence‐free survival and overall survival of high CREPT expression groups were significantly shorter than those of the low CREPT expression group. Multivariate analysis identified that CREPT might be an independent biomarker for the prediction of NSCLC prognosis. Overexpression of CREPT increased cell proliferation and enhanced the migration and invasion ability of Calu‐1 cells (a human NSCLC cell line with relative low CRPET expression) in vitro. Moreover, CREPT overexpression promoted tumor growth in a nude mice model. These results suggest that CREPT is closely relevant to the proliferation of NSCLC cells and it might be a potential prognostic marker in NSCLC patients.
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