Mechanical forces drive ordered patterning of hair cells in the mammalian inner ear.
Mechanical forces drive ordered patterning of hair cells in the mammalian inner ear.
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DOI：
10.1038/s41467-020-18894-8
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发表时间：
2020-10-12
影响因子：
16.6
通讯作者：
Sprinzak D
中科院分区：
文献类型：
--
作者：
Cohen R;Amir-Zilberstein L;Hersch M;Woland S;Loza O;Taiber S;Matsuzaki F;Bergmann S;Avraham KB;Sprinzak D
许多器官和组织的功能都需要细胞的周期性组织。这种周期性模式的发展通常与基于细胞间信号传导的机制相关，例如侧抑制和图灵模式。在这里，我们表明，从无序的过渡到有序的棋盘状图案的毛细胞和支持细胞在哺乳动物的听觉器官，Corti的器官，可能是基于机械力，而不是信号事件。使用小鼠耳蜗外植体的延时成像，我们表明，毛细胞通过协调嵌入和分层事件的组织范围的剪切运动逐渐重新排列成棋盘状图案。使用组织的力学模型，我们表明毛细胞上的全局剪切力和局部排斥力足以驱动从无序到有序的细胞模式转变。我们的研究结果表明，机械力驱动有序的毛细胞图案化的过程惊人地类似于聚合物和颗粒物理学中的剪切诱导结晶过程。细胞的周期性组织通常与基于细胞间信号传导的机制相关，例如侧抑制和图灵模式。在这里，作者表明，内耳毛细胞通过组织范围的剪切运动逐渐重新排列成棋盘状图案，协调嵌入和分层事件。
Periodic organization of cells is required for the function of many organs and tissues. The development of such periodic patterns is typically associated with mechanisms based on intercellular signaling such as lateral inhibition and Turing patterning. Here we show that the transition from disordered to ordered checkerboard-like pattern of hair cells and supporting cells in the mammalian hearing organ, the organ of Corti, is likely based on mechanical forces rather than signaling events. Using time-lapse imaging of mouse cochlear explants, we show that hair cells rearrange gradually into a checkerboard-like pattern through a tissue-wide shear motion that coordinates intercalation and delamination events. Using mechanical models of the tissue, we show that global shear and local repulsion forces on hair cells are sufficient to drive the transition from disordered to ordered cellular pattern. Our findings suggest that mechanical forces drive ordered hair cell patterning in a process strikingly analogous to the process of shear-induced crystallization in polymer and granular physics. The periodic organization of cells is typically associated with mechanisms based on intercellular signaling such as lateral inhibition and Turing patterning. Here the authors show that hair cells in the inner ear rearrange gradually into a checkerboard-like pattern through a tissue-wide shear motion that coordinates intercalation and delamination events.
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DOI：
10.1083/jcb.201509020
发表时间：
2016-02-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Katsunuma S;Honda H;Shinoda T;Ishimoto Y;Miyata T;Kiyonari H;Abe T;Nibu K;Takai Y;Togashi H
通讯作者：
Togashi H
影响因子：
9.2
作者：
Ebrahim, Seham;Fujita, Tomoki;Millis, Bryan A.;Kozin, Elliott;Ma, Xuefei;Kawamoto, Sachiyo;Baird, Michelle A.;Davidson, Michael;Yonemura, Shigenobu;Hisa, Yasuo;Conti, Mary Anne;Adelstein, Robert S.;Sakaguchi, Hirofumi;Kachar, Bechara
通讯作者：
Kachar, Bechara
影响因子：
4.3
作者：
Chiou, Kevin K.;Hufnagel, Lars;Shraiman, Boris I.
通讯作者：
Shraiman, Boris I.
影响因子：
2.4
作者：
Basch ML;Brown RM 2nd;Jen HI;Groves AK
通讯作者：
Groves AK
影响因子：
5.7
作者：
Kelly MC;Chen P
通讯作者：
Chen P