Pericyte-based human tissue engineered vascular grafts.
Pericyte-based human tissue engineered vascular grafts.
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DOI：
10.1016/j.biomaterials.2010.07.034
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发表时间：
2010-11
期刊：
影响因子：
14
通讯作者：
Vorp, David A.
中科院分区：
文献类型：
--
作者：
He, Wei;Nieponice, Alejandro;Soletti, Lorenzo;Hong, Yi;Gharaibeh, Burhan;Crisan, Mihaela;Usas, Arvydas;Peault, Bruno;Huard, Johnny;Wagner, William R.;Vorp, David A.
小直径组织工程血管移植物(TEVGs)的成功很大程度上依赖于合适的细胞来源和高效的细胞输送和载体系统。最近发现周细胞表达间充质干细胞的特征。它们的相对可用性和多潜能使它们成为TEVG应用的有前途的候选者。本研究的目的是将周细胞快速、密集、高效地整合到可生物降解支架中，并评价周细胞接种支架在体内的效果。用自制的旋转式真空播种器，将长度为10 mm、内径为1.3 mm的双层弹性聚酯氨酯尿素支架与3×106个周细胞混合种植，播种效率为90%。将接种的支架在旋转瓶中培养2d，然后作为主动脉间置移植物植入Lewis大鼠体内8周。结果表明，经动态培养后，周细胞均匀填充于支架的多孔层，并保持其原有的表型。植入后，种植周细胞的TEVGs的通畅率明显高于未种植的对照组：100%比38%(p<；0.05)。专利周细胞种植的TEVGs显示广泛的组织重塑，胶原和弹性蛋白存在。重建的组织由多层α--平滑肌肌动蛋白和钙蛋白阳性细胞以及管腔内一层von Willebrand因子阳性的单层细胞组成。这些结果证明了以周细胞为基础的TEVG的可行性，并提示周细胞在维持TEVG作为动脉管道的通畅性方面发挥了作用。
The success of small-diameter tissue engineered vascular grafts (TEVGs) greatly relies on an appropriate cell source and an efficient cellular delivery and carrier system. Pericytes have recently been shown to express mesenchymal stem cell features. Their relative availability and multipotentiality make them a promising candidate for TEVG applications. The objective of this study was to incorporate pericytes into a biodegradable scaffold rapidly, densely and efficiently, and to assess the efficacy of the pericyte-seeded scaffold in vivo. Bi-layered elastomeric poly(ester-urethane)urea scaffolds (length=10mm; inner diameter=1.3mm) were bulk seeded with 3×106 pericytes using a customized rotational vacuum seeding device in less than 2 min (seeding efficiency > 90%). The seeded scaffolds were cultured in spinner flasks for 2 days and then implanted into Lewis rats as aortic interposition grafts for 8 weeks. Results showed pericytes populated the porous layer of the scaffolds evenly and maintained their original phenotype after the dynamic culture. After implantation, pericyte-seeded TEVGs showed a significant higher patency rate than the unseeded control: 100% versus 38% (p < 0.05). Patent pericyte-seeded TEVGs revealed extensive tissue remodeling with collagen and elastin present. The remodeled tissue consisted of multiple layers of α-smooth muscle actin- and calponin-positive cells, and a von Willebrand factor-positive monolayer in the lumen. These results demonstrate the feasibility of a pericyte-based TEVG and suggest that the pericytes play a role in maintaining patency of the TEVG as an arterial conduit.
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DOI：
10.1016/j.jtcvs.2009.09.057
发表时间：
2010-02-01
影响因子：
6
作者：
Hibino, Narutoshi;McGillicuddy, Edward;Shinoka, Toshiharu
通讯作者：
Shinoka, Toshiharu
影响因子：
1
作者：
de Mel, Achala;Punshon, Geoffrey;Seifalian, Alexander M.
通讯作者：
Seifalian, Alexander M.
DOI：
10.1016/j.jtcvs.2004.10.030
发表时间：
2005-05-01
影响因子：
6
作者：
Hibino, N;Shin'oka, T;Kurosawa, H
通讯作者：
Kurosawa, H
DOI：
10.1073/pnas.0911465107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Roh, Jason D.;Sawh-Martinez, Rajendra;Breuer, Christopher K.
通讯作者：
Breuer, Christopher K.
DOI：
10.1002/jbm.10204
发表时间：
2002-09-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Guan, JJ;Sacks, MS;Wagner, WR
通讯作者：
Wagner, WR