Spleen glia are a transcriptionally unique glial subtype interposed between immune cells and sympathetic axons.
Spleen glia are a transcriptionally unique glial subtype interposed between immune cells and sympathetic axons.
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DOI：
10.1002/glia.23993
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发表时间：
2021-07
期刊：
影响因子：
6.2
通讯作者：
Buckwalter MS
中科院分区：
文献类型：
--
作者：
Lucas TA;Zhu L;Buckwalter MS
已知神经胶质在大脑、肠道和坐骨神经周围发挥重要作用。虽然肠道有自己专门的神经系统，但其他内脏仅由自主神经支配。虽然神经胶质细胞是周围神经系统中最丰富的细胞类型之一，但其伴随自主神经支配的功能还不清楚。在这里，我们专注于脾脏中的非髓鞘化雪旺细胞，脾脏胶质细胞。脾是由交感神经系统支配的主要免疫器官，其调节免疫功能。这种相互作用被称为神经免疫通讯。我们建立了脾胶质细胞可以可视化使用免疫组化S100B和GFAP和报告小鼠。脾神经胶质包裹交感神经轴突，位于脾富含淋巴细胞的白色髓区。我们对脾脏胶质细胞转录组进行了测序，并确定了可能参与轴突鞘化以及与神经和免疫细胞通讯的基因。脾神经胶质细胞表达交感神经轴突（肾上腺素能、嘌呤能和神经肽Y）产生的神经递质受体，以及细胞因子、趋化因子及其受体，这些受体可能与脾中的免疫细胞通讯。我们还建立了脾脏胶质细胞和其他胶质细胞类型之间的相似性和差异。虽然所有神经胶质细胞共享许多共同的基因，但脾神经胶质细胞差异表达与免疫应答相关的基因，包括参与嘌呤-细胞因子受体相互作用、吞噬作用和补体级联反应的基因。因此，脾神经胶质细胞是一种独特的神经胶质细胞类型，在物理和转录上准备参与脾中的神经免疫通讯。
Glia are known to play important roles in the brain, the gut, and around the sciatic nerve. While the gut has its own specialized nervous system, other viscera are innervated solely by autonomic nerves. The functions of glia that accompany autonomic innervation are not well known, even though they are one of the most abundant cell types in the peripheral nervous system. Here, we focused on non-myelinating Schwann cells in the spleen, spleen glia. The spleen is a major immune organ innervated by the sympathetic nervous system, which modulates immune function. This interaction is known as neuroimmune communication. We establish that spleen glia can be visualized using both immunohistochemistry for S100B and GFAP and with a reporter mouse. Spleen glia ensheath sympathetic axons and are localized to the lymphocyte-rich white pulp areas of the spleen. We sequenced the spleen glia transcriptome and identified genes that are likely involved in axonal ensheathment and communication with both nerves and immune cells. Spleen glia express receptors for neurotransmitters made by sympathetic axons (adrenergic, purinergic, and Neuropeptide Y), and also cytokines, chemokines, and their receptors that may communicate with immune cells in the spleen. We also established similarities and differences between spleen glia and other glial types. While all glia share many genes in common, spleen glia differentially express genes associated with immune responses, including genes involved in cytokine-cytokine receptor interactions, phagocytosis, and the complement cascade. Thus, spleen glia are a unique glial type, physically and transcriptionally poised to participate in neuroimmune communication in the spleen.
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通讯作者：
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10.1152/ajpgi.00155.2018
发表时间：
2018-10-01
影响因子：
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作者：
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