The Effects of Graded Levels of Calorie Restriction: X. Transcriptomic Responses of Epididymal Adipose Tissue.
The Effects of Graded Levels of Calorie Restriction: X. Transcriptomic Responses of Epididymal Adipose Tissue.
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DOI：
10.1093/gerona/glx101
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发表时间：
2018-03-02
期刊：
影响因子：
--
通讯作者：
Speakman JR
中科院分区：
文献类型：
--
作者：
Derous D;Mitchell SE;Green CL;Wang Y;Han JDJ;Chen L;Promislow DEL;Lusseau D;Douglas A;Speakman JR
热量限制（CR）导致脂肪组织质量显着减少，并增加许多类群的寿命。自从发现瘦素以来，脂肪组织的分泌能力在对CR的反应中得到了突出。我们对暴露于分级CR水平（0- 40%CR）3个月的雄性C57 BL/6小鼠附睾白色脂肪组织的转录物进行定量。NF-κB、HIF 1-α和p53信号转导相关的差异表达基因（DEG）数量随着CR水平的增加而增加。相对于12小时随意进食，这些途径在40%CR时均显着下调。此外，分级CR对与血管生成相关的通路相关的DEG有实质性影响。在CR分级差异表达的497个基因中，其中155个基因包含信号肽基序。这些推定的信号蛋白参与对酮的反应、TGF-β信号传导、胰岛素分泌的负调节和炎症。这与先前确定的分级CR对相同小鼠中葡萄糖稳态的影响一致。总的来说，这些数据表明，CR下脂肪组织水平的降低可能以多种方式促进CR的保护作用，这些方式与大量分泌蛋白的变化有关。
Calorie restriction (CR) leads to a remarkable decrease in adipose tissue mass and increases longevity in many taxa. Since the discovery of leptin, the secretory abilities of adipose tissue have gained prominence in the responses to CR. We quantified transcripts of epididymal white adipose tissue of male C57BL/6 mice exposed to graded levels of CR (0–40% CR) for 3 months. The numbers of differentially expressed genes (DEGs) involved in NF-κB, HIF1-α, and p53 signaling increased with increasing levels of CR. These pathways were all significantly downregulated at 40% CR relative to 12 h ad libitum feeding. In addition, graded CR had a substantial impact on DEGs associated with pathways involved in angiogenesis. Of the 497 genes differentially expressed with graded CR, 155 of these genes included a signal peptide motif. These putative signaling proteins were involved in the response to ketones, TGF-β signaling, negative regulation of insulin secretion, and inflammation. This accords with the previously established effects of graded CR on glucose homeostasis in the same mice. Overall these data suggest reduced levels of adipose tissue under CR may contribute to the protective impact of CR in multiple ways linked to changes in a large population of secreted proteins.
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DOI：
10.18632/aging.100895
发表时间：
2016-04
期刊：
Aging
影响因子：
--
作者：
Derous D;Mitchell SE;Green CL;Chen L;Han JD;Wang Y;Promislow DE;Lusseau D;Speakman JR;Douglas A
通讯作者：
Douglas A
影响因子：
120.1
作者：
Cao, Yihai
通讯作者：
Cao, Yihai
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
--
作者：
Fontana, Luigi;Klein, Samuel;Holloszy, John O.
通讯作者：
Holloszy, John O.
DOI：
10.1073/pnas.91.15.7036
发表时间：
1994-07-19
影响因子：
11.1
作者：
HURSTING, SD;PERKINS, SN;PHANG, JM
通讯作者：
PHANG, JM