Abscission is regulated by the ESCRT-III protein shrub in Drosophila germline stem cells.
Abscission is regulated by the ESCRT-III protein shrub in Drosophila germline stem cells.
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DOI：
10.1371/journal.pgen.1004653
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发表时间：
2015-02
期刊：
影响因子：
4.5
通讯作者：
Huynh JR
中科院分区：
文献类型：
--
作者：
Matias NR;Mathieu J;Huynh JR
脱落是胞质分裂的最后一个事件，导致两个子细胞的物理分离。最近的技术进步使人们能够更好地了解导致分离的酵母或哺乳动物细胞中的凋亡的细胞和分子事件。然而，在不同的细胞类型或发育中的生物体中，如何调节凋亡仍然知之甚少。在这里，我们的特点是ESCRT-III蛋白灌木在细胞质分裂过程中的生殖细胞经历了一系列的完整和不完整的分裂功能。我们发现，灌木是需要完全解除，和灌木的水平是至关重要的适当时机解除。Shrub的丧失或获得延迟了生殖系干细胞（GSC）的分裂，并导致干细胞囊肿的形成，其中子细胞与母干细胞共享相同的细胞质，并且不能分化。此外，我们的研究结果表明，极光B激酶在GSC凋亡的灌木负调控。最后，我们发现，致死巨盘（lgd），已知是所需的灌木功能的内体途径，也调节GSC的持续时间。脱落是胞质分裂的最后一步，通过有丝分裂结束时连接姐妹细胞的细胞质桥的断裂，使姐妹细胞物理分离。脱落的持续时间取决于细胞类型，表明该事件是发育调节的。最近，我们已经确定了两种激酶，极光B和CycB/Cdk-1，它们调节生殖细胞和哺乳动物细胞中的脱落时间。然而，这些激酶是上游调节剂，本身不进行水解。在这里，我们表明，灌木，极光B的一个潜在的目标和最下游的效应器之一，在生殖系干细胞中的完全脱落。在没有灌木的情况下，母干细胞仍然与其子细胞相连，然后共享相同的细胞质并且不能分化。Shrub和Aurora B的缺失对休眠持续时间有相反的影响，表明Aurora B负调节Shrub。我们进一步表明，灌木的行为与其相互作用致命的巨大光盘，以确保适当的终止时间。
Abscission is the final event of cytokinesis that leads to the physical separation of the two daughter cells. Recent technical advances have allowed a better understanding of the cellular and molecular events leading to abscission in isolated yeast or mammalian cells. However, how abscission is regulated in different cell types or in a developing organism remains poorly understood. Here, we characterized the function of the ESCRT-III protein Shrub during cytokinesis in germ cells undergoing a series of complete and incomplete divisions. We found that Shrub is required for complete abscission, and that levels of Shrub are critical for proper timing of abscission. Loss or gain of Shrub delays abscission in germline stem cells (GSCs), and leads to the formation of stem-cysts, where daughter cells share the same cytoplasm as the mother stem cell and cannot differentiate. In addition, our results indicate a negative regulation of Shrub by the Aurora B kinase during GSC abscission. Finally, we found that Lethal giant discs (lgd), known to be required for Shrub function in the endosomal pathway, also regulates the duration of abscission in GSCs. Abscission is the final step of cytokinesis which allows the physical separation of sister cells through the scission of a thin cytoplasmic bridge that links them at the end of mitosis. The duration of abscission varies depending on cell types, indicating that the event is developmentally regulated. Recently, we have identified two kinases, Aurora B and CycB/Cdk-1, which regulate the timing of abscission in germ cells and in mammalian cells. However, these kinases are upstream regulators and do not perform abscission per se. Here, we show that Shrub, a potential target of Aurora B and one of the most downstream effectors of abscission, is required for complete abscission in germline stem cells. In the absence of Shrub, the mother stem cell remains linked to its daughter cells, which then share the same cytoplasm and cannot differentiate. Loss of Shrub and Aurora B have opposite effects on abscission duration suggesting that Aurora B regulates negatively Shrub. We further show that Shrub acts together with its interactor Lethal giant disc to ensure proper abscission timing.
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影响因子：
9.2
作者：
Childress, Jennifer L.;Acar, Melih;Haider, Georg
通讯作者：
Haider, Georg
影响因子：
56.9
作者：
Carlton, Jez G.;Martin-Serrano, Juan
通讯作者：
Martin-Serrano, Juan
影响因子：
11.8
作者：
Gallagher, Ciara M.;Knoblich, Juergen A.
通讯作者：
Knoblich, Juergen A.
影响因子：
56.9
作者：
Guizetti, Julien;Schermelleh, Lothar;Gerlich, Daniel W.
通讯作者：
Gerlich, Daniel W.
DOI：
10.1083/jcb.201008108
发表时间：
2010-11-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bastos RN;Barr FA
通讯作者：
Barr FA