Viral genome sequencing by random priming methods.
Viral genome sequencing by random priming methods.
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DOI：
10.1186/1471-2164-9-5
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发表时间：
2008-01-07
期刊：
影响因子：
4.4
通讯作者：
Spiro, David J.
中科院分区：
文献类型：
--
作者：
Djikeng, Appolinaire;Halpin, Rebecca;Kuzmickas, Ryan;DePasse, Jay;Feldblyum, Jeremy;Sengamalay, Naomi;Afonso, Claudio;Zhang, Xinsheng;Anderson, Norman G.;Ghedin, Elodie;Spiro, David J.
大多数新出现的健康威胁都源于人畜共患疾病。对于绝大多数，它们的病原体是RNA病毒，包括但不限于HIV、流感、SARS、埃博拉、登革热和汉坦病毒。因此，更好地了解全球病毒多样性以更好地监测和预测大流行威胁变得越来越重要;这将需要快速和灵活的方法来完成病毒基因组测序。我们已经将SISPA方法用于RNA和DNA病毒的基因组测序。我们已经证明了该方法在各种类型和来源的病毒上的实用性，获得了大小为3，000 - 15，000 kb的病毒的接近完整的基因组序列，覆盖范围的中值深度为14.33。我们使用该技术在宿主污染物存在下，使用来自细胞培养上清液、尿囊液和粪便的病毒制备物来产生全病毒基因组序列。所描述的方法对于生成具有很少或没有可用序列信息的病毒、来自截然不同家族的病毒、以前未表征的病毒的全基因组组装或更全面地描述混合病毒感染非常有用。
Most emerging health threats are of zoonotic origin. For the overwhelming majority, their causative agents are RNA viruses which include but are not limited to HIV, Influenza, SARS, Ebola, Dengue, and Hantavirus. Of increasing importance therefore is a better understanding of global viral diversity to enable better surveillance and prediction of pandemic threats; this will require rapid and flexible methods for complete viral genome sequencing. We have adapted the SISPA methodology to genome sequencing of RNA and DNA viruses. We have demonstrated the utility of the method on various types and sources of viruses, obtaining near complete genome sequence of viruses ranging in size from 3,000–15,000 kb with a median depth of coverage of 14.33. We used this technique to generate full viral genome sequence in the presence of host contaminants, using viral preparations from cell culture supernatant, allantoic fluid and fecal matter. The method described is of great utility in generating whole genome assemblies for viruses with little or no available sequence information, viruses from greatly divergent families, previously uncharacterized viruses, or to more fully describe mixed viral infections.
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影响因子：
56.9
作者：
Venter, JC;Remington, K;Smith, HO
通讯作者：
Smith, HO
DOI：
10.1073/pnas.0504666102
发表时间：
2005-09-06
影响因子：
11.1
作者：
Allander, T;Tammi, MT;Andersson, B
通讯作者：
Andersson, B
影响因子：
5.4
作者：
Jones, MS;Kapoor, A;Delwart, E
通讯作者：
Delwart, E
影响因子：
64.8
作者：
Ghedin, E;Sengamalay, NA;Salzberg, SL
通讯作者：
Salzberg, SL
影响因子：
82.9
作者：
van der Hoek L;Pyrc K;Jebbink MF;Vermeulen-Oost W;Berkhout RJ;Wolthers KC;Wertheim-van Dillen PM;Kaandorp J;Spaargaren J;Berkhout B
通讯作者：
Berkhout B