Ammonia oxidizers and denitrifiers in response to reciprocal elevation translocation in an alpine meadow on the Tibetan Plateau
Ammonia oxidizers and denitrifiers in response to reciprocal elevation translocation in an alpine meadow on the Tibetan Plateau
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青藏高原高寒草甸的氨氧化剂和反硝化菌对相互海拔易位的响应
DOI：
10.1007/s11368-014-0867-7
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发表时间：
2014-02
影响因子：
3.6
通讯作者：
Liang-Dong Guo
中科院分区：
文献类型：
--
作者：
Yong Zheng;Wei Yang;Hang-Wei Hu;Yong-Chan Kim;Ji-Chuan Duan;Cai-Yun Luo;Shi-Ping Wang;Liang-Dong Guo
全球气候变化，特别是气温变化，可能改变土壤微生物的丰度和组成，从而影响土壤地球化学循环和生态系统功能。然而，地下氮转化微生物对高海拔陆地生态系统温度变化的响应还没有得到很好的理解，材料和方法在这里，模拟冷却和升温对氨氧化古菌（AOA）和细菌（AOB）的丰度和群落组成的影响，以及丰富的硝化细菌，采用定量聚合酶链反应和克隆文库方法，通过对青藏高原海拔3200 ~ 3800 m的海拔梯度进行为期沿着2年的反向迁移试验，结果表明，与海拔迁移引起的温度变化相比，土壤来源对AOA丰度的影响更大。土壤来源和海拔迁移对AOB丰度均有显著影响，海拔升高（模拟降温）处理降低AOB丰度，海拔降低（模拟升温）处理增加AOB丰度。温度的变化影响nirKrather基因的丰度，而nirSandnosZ基因的丰度，后两者似乎与土壤来源密切相关。温度变化对氨氧化细菌群落结构和多样性有明显影响，对氨氧化细菌群落结构和多样性影响不大。AOB对气候变暖的响应比其他研究中的草地和森林生态系统更明显，这可能是由于海拔较高，年平均温度较低，在本study.ConclusionsOur研究结果表明，AOB相比AOA和退化种群，可能对海拔迁移引起的自然温度变化更敏感，在青藏高原高寒草地生态系统。
PurposeGlobal climate change, in particular temperature variation, is likely to alter soil microbial abundance and composition, with consequent impacts on soil biogeochemical cycling and ecosystem functioning. However, responses of belowground nitrogen transformation microorganisms to temperature changes in high-elevation terrestrial ecosystems are not well understood.Materials and methodsHere, the effects of simulated cooling and warming on the abundance and community composition of ammonia-oxidizing archaea (AOA) and bacteria (AOB), as well as the abundance of denitrifiers, were investigated using quantitative polymerase chain reaction and clone library approaches, on the basis of a 2-year reciprocal elevation translocation experiment along an elevation gradient from 3,200 to 3,800 m above sea level on the Tibetan Plateau.Results and discussionWe found that, compared with the temperature variations caused by elevation translocation, the soil origin exerted a much stronger influence on AOA abundance. There were significant effects of both soil origin and elevation translocation on AOB abundance, which was particularly decreased by elevation-enhanced (simulated cooling) and increased by elevation-decreased (simulated warming) treatments. Altered temperature affected the abundance ofnirKrather thannirSandnosZgenes, and the latter two seemed to be associated tightly with the soil origin. Furthermore, the results showed that temperature changes had obvious influences on the community structure and diversity of AOB, but not AOA. More apparent response of AOB to warming than in other studies on grassland and forest ecosystems may be attributed to higher elevation and lower mean annual temperature in this study.ConclusionsOur findings thus suggest that, in comparison with AOA and denitrifying populations, AOB may respond more sensitively to natural temperature variation caused by elevation translocation in this alpine grassland ecosystem on the Tibetan Plateau.
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影响因子：
4.2
作者：
Szukics U;Abell GC;Hödl V;Mitter B;Sessitsch A;Hackl E;Zechmeister-Boltenstern S
通讯作者：
Zechmeister-Boltenstern S
影响因子：
2.9
作者：
Yucheng Wu;Lu Lu-Lu;Baozhan Wang;Xiangui Lin;Jianguo Zhu;Z. Cai;Xiaoyuan Yan;Z. Jia
通讯作者：
Yucheng Wu;Lu Lu-Lu;Baozhan Wang;Xiangui Lin;Jianguo Zhu;Z. Cai;Xiaoyuan Yan;Z. Jia
DOI：
10.1098/rstb.2010.0038
发表时间：
2010-07
期刊：
Philosophical Transactions of the Royal Society B: Biological Sciences
影响因子：
--
作者：
G. Yvon‐Durocher;J. I. Jones;M. Trimmer;G. Woodward;J. Montoya;J. Montoya
通讯作者：
G. Yvon‐Durocher;J. I. Jones;M. Trimmer;G. Woodward;J. Montoya;J. Montoya
影响因子：
5.2
作者：
Daebeler A;Abell GC;Bodelier PL;Bodrossy L;Frampton DM;Hefting MM;Laanbroek HJ
通讯作者：
Laanbroek HJ
DOI：
10.1038/ismej.2008.52
发表时间：
2008-06
期刊：
The ISME Journal
影响因子：
--
作者：
Jennifer K. M. Walker;K. Egger;G. Henry
通讯作者：
Jennifer K. M. Walker;K. Egger;G. Henry