Archaeal dominated ammonia-oxidizing communities in Icelandic grassland soils are moderately affected by long-term N fertilization and geothermal heating.
Archaeal dominated ammonia-oxidizing communities in Icelandic grassland soils are moderately affected by long-term N fertilization and geothermal heating.
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DOI：
10.3389/fmicb.2012.00352
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发表时间：
2012
影响因子：
5.2
通讯作者：
Laanbroek HJ
中科院分区：
文献类型：
--
作者：
Daebeler A;Abell GC;Bodelier PL;Bodrossy L;Frampton DM;Hefting MM;Laanbroek HJ
氨氧化细菌和古细菌（分别为 AOB 和 AOA）对氨净氧化的贡献在陆地环境之间存在很大差异。为了更好地理解、预测和管理陆地氮周转，我们需要对氨氧化作为环境条件（包括相关生物的生态生理学）的函数有一个概念性的理解。我们通过沿着冰岛草原温度梯度的长期施肥地点的土壤取样，研究了矿物氮沉降和地热加热对氨氧化群落的离散和综合影响。对氨单加氧酶亚基 A (amoA) 基因进行了微阵列、克隆文库和定量 PCR 分析，同时对土壤特性进行了物理化学测量。与大多数其他陆地环境相比，氨氧化群落几乎完全由古细菌组成。定量聚合酶链式反应无法检测到它们的细菌对应物，这表明 AOB 与这些土壤中的氨氧化关系不大。我们的结果表明，施肥和局部地热变暖影响可检测到的氨氧化群落，但不影响土壤化学：在较高温度的土壤中仅存在检测到的AOA系统型的一个子集，并且施肥土壤中AOA丰度增加，而土壤理化性质保持不变。 AOA群落分布和结构的差异最好由土壤pH值和粘土含量来解释，与这些草原土壤的温度或肥料处理无关，这表明这些因素比温度和施氮肥对土壤中AOA的生态位分化具有更大的潜力。
The contribution of ammonia-oxidizing bacteria and archaea (AOB and AOA, respectively) to the net oxidation of ammonia varies greatly between terrestrial environments. To better understand, predict and possibly manage terrestrial nitrogen turnover, we need to develop a conceptual understanding of ammonia oxidation as a function of environmental conditions including the ecophysiology of associated organisms. We examined the discrete and combined effects of mineral nitrogen deposition and geothermal heating on ammonia-oxidizing communities by sampling soils from a long-term fertilization site along a temperature gradient in Icelandic grasslands. Microarray, clone library and quantitative PCR analyses of the ammonia monooxygenase subunit A (amoA) gene accompanied by physico-chemical measurements of the soil properties were conducted. In contrast to most other terrestrial environments, the ammonia-oxidizing communities consisted almost exclusively of archaea. Their bacterial counterparts proved to be undetectable by quantitative polymerase chain reaction suggesting AOB are only of minor relevance for ammonia oxidation in these soils. Our results show that fertilization and local, geothermal warming affected detectable ammonia-oxidizing communities, but not soil chemistry: only a subset of the detected AOA phylotypes was present in higher temperature soils and AOA abundance was increased in the fertilized soils, while soil physio-chemical properties remained unchanged. Differences in distribution and structure of AOA communities were best explained by soil pH and clay content irrespective of temperature or fertilizer treatment in these grassland soils, suggesting that these factors have a greater potential for ecological niche-differentiation of AOA in soil than temperature and N fertilization.
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影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
2.3
作者：
Ijichi, Minoru;Hamasaki, Koji
通讯作者：
Hamasaki, Koji
DOI：
10.1073/pnas.1109000108
发表时间：
2011-12-27
影响因子：
11.1
作者：
Gubry-Rangin, Cecile;Hai, Brigitte;Nicol, Graeme W.
通讯作者：
Nicol, Graeme W.
影响因子：
3.6
作者：
Fan, Fenliang;Yang, Qianbao;Liang, Yongchao
通讯作者：
Liang, Yongchao
影响因子：
4.2
作者：
Di, Hong J.;Cameron, Keith C.;He, Ji-Zheng
通讯作者：
He, Ji-Zheng