Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
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马克斯克鲁维酵母 NBRC1777 木糖代谢途径中催化木酮糖磷酸化的木酮糖激酶的鉴定
DOI：
10.1007/s10295-011-0963-2
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发表时间：
2011-03
影响因子：
3.4
通讯作者：
Hong, Jiong
中科院分区：
文献类型：
--
作者：
Wang, Rongliang;Zhang, Ling;Wang, Dongmei;Gao, Xiaolian;Hong, Jiong
木聚糖酶是木糖代谢和发酵过程中的关键酶之一，通过微调木聚糖酶的表达可以提高酵母中木糖的发酵。为了提高马氏克鲁维菌木糖发酵效率，从马氏克鲁维菌NBRC1777中分离到了编码D：-木聚糖酶(E.C.2.7.1.17)的基因KmXYL3。在大肠杆菌BL21(DE3)中表达了KmXYL3，纯化后的重组木聚糖酶的比活力为23.5mU/mg。KmXYL3基因的缺失导致了木糖醇利用的丧失和木糖利用的显著降低，证明KmXYL3编码一种木聚糖酶，在木糖代谢途径中催化木果糖转化为木果糖5-磷酸的反应。在KmXYL3中断菌株中观察到木糖的缓慢同化，这表明KmXYL3对木糖和木糖醇的利用是关键的；然而，马氏克氏酵母利用旁路进行木糖同化，这一途径不涉及木糖醇或木果糖。
Xylulokinase is one of the key enzymes in xylose metabolism and fermentation, and fine-tuned expression of xylulokinase can improve xylose fermentation in yeast. To improve the efficiency of xylose fermentation in Kluyveromyces marxianus, the gene KmXYL3, which encodes a D: -xylulokinase (E.C. 2.7.1.17), was isolated from K. marxianus NBRC1777. KmXYL3 was expressed in Escherichia coli BL21 (DE3) cells, and the specific activity of the resulting recombinant purified xylulokinase was 23.5 mU/mg. Disruption of KmXYL3 resulted in both loss of xylitol utilization and marked decrease in xylose utilization, proving that KmXYL3 encodes a xylulokinase that catalyzes the reaction from xylulose to xylulose 5-phosphate in the xylose metabolic pathway. The slow assimilation of xylose observed in the KmXYL3-disrupted strain indicates that KmXYL3 is critical for xylose and xylitol utilization; however, K. marxianus utilizes a bypass pathway for xylose assimilation, and this pathway does not involve xylitol or xylulose.
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影响因子：
5
作者：
Hong, Jiong;Tamaki, Hisanori;Kumagai, Hidehiko
通讯作者：
Kumagai, Hidehiko
影响因子：
2.1
作者：
Rodriguez-Peña, JM;Cid, VJ;Nombela, C
通讯作者：
Nombela, C
DOI：
10.1073/pnas.89.16.7290
发表时间：
1992-08-15
影响因子：
11.1
作者：
BORK, P;SANDER, C;VALENCIA, A
通讯作者：
VALENCIA, A
影响因子：
4.4
作者：
Eliasson, A;Christensson, C;Hahn-Hägerdal, B
通讯作者：
Hahn-Hägerdal, B
影响因子：
4.4
作者：
P. Stevis;James J. Huang;N. Ho
通讯作者：
P. Stevis;James J. Huang;N. Ho