Lasiodiplodia theobromae protein LtScp1 contributes to fungal virulence and protects fungal mycelia against hydrolysis by grapevine chitinase.
Lasiodiplodia theobromae protein LtScp1 contributes to fungal virulence and protects fungal mycelia against hydrolysis by grapevine chitinase.
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可可毛二孢蛋白 LtScp1 有助于真菌毒力并保护真菌菌丝体免受葡萄几丁质酶的水解
DOI：
10.1111/1462-2920.16155
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发表时间：
2022-10
影响因子：
5.1
通讯作者：
Yan, Jiye
中科院分区：
文献类型：
--
作者：
Peng, Junbo;Li, Xinghong;Li, Yonghua;Zhang, Wei;Zhou, Ying;Yan, Jiye
摘要 据报道，LysM 蛋白对于草本植物病原体的毒力和宿主免疫抑制非常重要，而木本植物病原体 Lasiodiplodia theobromae 的信息却少之又少。为了研究L. theobromae中LysM蛋白的功能机制，本研究克隆并详细表征了一个基因LtScp1。转录分析显示 LtScp1 在感染阶段高度表达。与野生型相比，可可乳杆菌中 LtScp1 的过度表达和沉默分别导致病变区域显着增加和减少。此外，通过酵母信号肽捕获系统，LtScp1 被确定为分泌蛋白。有趣的是，LtScp1 被证实被 N-糖基化修饰，这对于 LtScp1 分子的同二聚化是必需的。此外，还发现LtScp1与葡萄几丁质酶VvChi4相互作用并干扰VvChi4结合几丁质的能力。总的来说，这些结果表明 LtScp1 作为毒力因子发挥作用，保护真菌在感染过程中免遭降解。
Abstract The LysM proteins have been reported to be important for the virulence and host immunity suppression in herbaceous plant pathogens, whereas far less information is documented in the woody plant pathogen Lasiodiplodia theobromae. To investigate the functional mechanism of LysM protein in L. theobromae, one gene LtScp1 was cloned and characterized detailedly in the current study. Transcription profiling revealed that LtScp1 was highly expressed at the infectious stages. Compared to wild type, overexpression and silencing of LtScp1 in L. theobromae led to significantly increased and decreased lesion areas, respectively. Moreover, LtScp1 was determined to be a secreted protein via a yeast signal peptide trapping system. Interestingly, LtScp1 was confirmed to be modified by the N‐glycosylation, which is necessary for the homodimerization of LtScp1 molecules. Furthermore, it was found that LtScp1 interacted with the grapevine chitinase VvChi4 and interfered the ability of VvChi4 to bind chitin. Collectively, these results suggest that LtScp1 functions as a virulence factor to protect the fungus from degradation during the infection.
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影响因子：
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作者：
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DOI：
10.1073/pnas.85.3.782
发表时间：
1988-02-01
影响因子：
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作者：
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