Porcine epidemic diarrhea virus RNA present in commercial spray-dried porcine plasma is not infectious to naïve pigs.
Porcine epidemic diarrhea virus RNA present in commercial spray-dried porcine plasma is not infectious to naïve pigs.
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DOI：
10.1371/journal.pone.0104766
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Halbur PG
中科院分区：
文献类型：
--
作者：
Opriessnig T;Xiao CT;Gerber PF;Zhang J;Halbur PG
猪流行性腹泻病毒于2013年4月在北美出现，此后已在美国30个州、加拿大和墨西哥发现。PEDV的快速传播引起了人们对饲料，特别是猪肉副产品成分，如喷雾干燥猪血浆(SDPP)在PEDV传播中所起作用的担忧。本研究的目的是确定商业SDPP中存在的PEDV RNA的传染性。具体地说，40头3周龄的PEDV幼稚猪被随机分配到五个处理组中。在接种后第0天，NEG对照组猪进行假接种，PEDV对照组猪接受PEDV增殖细胞培养，SDPP对照组猪饲喂含有5.1±0.1log10个PEDV RNA拷贝/g的5%SDPP。为了评价SDPP中抗PEDV抗体对PEDV攻击的潜在积极作用，在PEDV攻击前4天，SDPP-PEDV组猪通过dpi 28切换到并保持5%SDPP饲料。另一组，鸡蛋-PEDV，口服商品鸡蛋衍生的液体PEDV球蛋白产品，从dpi-4到6。所有PEDV对照组的猪开始通过dpi 3从粪便中排出PEDV，并在dpi 7-14之间血清转换，而NEG对照组和SDPP对照组的猪在研究期间保持PEDV RNA阴性，没有血清转换为PEDV。这表明在使用的SDPP批次中没有证据表明PEDV RNA具有传染性。此外，在研究条件下，添加SDPP或鸡蛋衍生的液体PEDV球蛋白并不显著改变PEDV的脱落或在实验挑战后的总体病程。
Porcine epidemic diarrhea virus emerged in North America in April 2013 and has since been identified in 30 U.S. States, Canada and Mexico. The rapid spread of PEDV has raised concerns about the role of feed and particularly pork-by-product components such as spray-dried porcine plasma (SDPP) in PEDV transmission. The aim of this study was to determine the infectivity of PEDV RNA present in commercial SDPP. Specifically, 40 3-week-old PEDV naïve pigs were randomly assigned to one of five treatment groups. At day post inoculation (dpi) 0, NEG-CONTROL pigs were sham-inoculated, PEDV-CONTROL pigs received cell culture propagated PEDV, and SDPP-CONTROL pigs were switched to a diet with 5% SDPP containing 5.1±0.1 log10 PEDV RNA copies/g. To evaluate a potential positive effect of anti-PEDV antibodies in SDPP on PEDV challenge, four days prior to PEDV challenge the pigs in the SDPP-PEDV group were switched to and remained on a 5% SDPP diet through dpi 28. Another group, EGG-PEDV, was orally administered a commercial egg-derived liquid PEDV globulin product from dpi -4 through 6. All PEDV-CONTROL pigs began shedding PEDV in feces by dpi 3 and seroconverted between dpi 7 and 14, whereas pigs in NEG-CONTROL and SDPP-CONTROL groups remained PEDV RNA negative and did not seroconvert to PEDV for the study duration. This indicates no evidence of infectivity of the PEDV RNA in the SDPP lot utilized. Furthermore, under the study conditions SDPP or egg-derived liquid PEDV globulin addition did not significantly alter PEDV-shedding or overall disease course after experimental challenge.
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影响因子：
3.3
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