Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation.
Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation.
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DOI：
10.1371/journal.pone.0243984
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Yoshida M
中科院分区：
文献类型：
--
作者：
Umezawa K;Niikura M;Kojima Y;Goodell B;Yoshida M
褐腐真菌在生物炼制木材转化系统中具有巨大潜力，因为它们是针叶林中主要的木材分解者，并且拥有高效的木质纤维素降解系统。它们最初的木材降解机制被认为是由一个基于氧化自由基的系统组成，该系统与一个酶促糖化系统依次作用，但这个系统的完整分子机制尚未阐明。一些研究表明，褐腐真菌的木材降解机制在底物选择性方面具有多样性。密粘褶菌（Gloeophyllum trabeum）是研究最多的褐腐真菌种类之一，具有广泛的底物选择性，甚至可以降解一些草本植物。然而，这种广泛的底物特异性的基础了解甚少。在这项研究中，我们对在以葡萄糖、纤维素或日本柳杉（Cryptomeria japonica）为唯一碳源的培养基上生长的密粘褶菌进行了RNA - seq分析。与在葡萄糖上的基因表达相比，在纤维素上有1129个基因上调，在柳杉上有1516个基因上调。密粘褶菌在纤维素和柳杉培养基上上调的碳水化合物活性酶（CAZyme）基因包括糖苷水解酶家族12（GH12）、GH131、碳水化合物酯酶家族1（CE1）、辅助活性家族3亚家族1（AA3_1）、AA3_2、AA3_4和AA9，这是褐腐真菌一种新报道的表达模式。在柳杉培养基上萜烯合酶和细胞色素P450基因的上调表明这些基因产物在与螯合剂介导的芬顿反应相关的次生代谢产物产生中可能具有重要性。这些结果为密粘褶菌固有的木材降解机制以及褐腐机制的多样性提供了新的见解。
Brown rot fungi have great potential in biorefinery wood conversion systems because they are the primary wood decomposers in coniferous forests and have an efficient lignocellulose degrading system. Their initial wood degradation mechanism is thought to consist of an oxidative radical-based system that acts sequentially with an enzymatic saccharification system, but the complete molecular mechanism of this system has not yet been elucidated. Some studies have shown that wood degradation mechanisms of brown rot fungi have diversity in their substrate selectivity. Gloeophyllum trabeum, one of the most studied brown rot species, has broad substrate selectivity and even can degrade some grasses. However, the basis for this broad substrate specificity is poorly understood. In this study, we performed RNA-seq analyses on G. trabeum grown on media containing glucose, cellulose, or Japanese cedar (Cryptomeria japonica) as the sole carbon source. Comparison to the gene expression on glucose, 1,129 genes were upregulated on cellulose and 1,516 genes were upregulated on cedar. Carbohydrate Active enZyme (CAZyme) genes upregulated on cellulose and cedar media by G. trabeum included glycoside hyrolase family 12 (GH12), GH131, carbohydrate esterase family 1 (CE1), auxiliary activities family 3 subfamily 1 (AA3_1), AA3_2, AA3_4 and AA9, which is a newly reported expression pattern for brown rot fungi. The upregulation of both terpene synthase and cytochrome P450 genes on cedar media suggests the potential importance of these gene products in the production of secondary metabolites associated with the chelator-mediated Fenton reaction. These results provide new insights into the inherent wood degradation mechanism of G. trabeum and the diversity of brown rot mechanisms.
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影响因子：
6.3
作者：
Couturier M;Navarro D;Chevret D;Henrissat B;Piumi F;Ruiz-Dueñas FJ;Martinez AT;Grigoriev IV;Riley R;Lipzen A;Berrin JG;Master ER;Rosso MN
通讯作者：
Rosso MN
影响因子：
3.7
作者：
Bromann K;Toivari M;Viljanen K;Vuoristo A;Ruohonen L;Nakari-Setälä T
通讯作者：
Nakari-Setälä T
影响因子：
4.4
作者：
Cohen, R;Suzuki, MR;Hammel, KE
通讯作者：
Hammel, KE
影响因子：
3.4
作者：
Presley GN;Ndimba BK;Schilling JS
通讯作者：
Schilling JS
影响因子：
3.7
作者：
Jawallapersand P;Mashele SS;Kovačič L;Stojan J;Komel R;Pakala SB;Kraševec N;Syed K
通讯作者：
Syed K