Molecular basis for avirulence of spontaneous variants of Porphyromonas gingivalis: Genomic analysis of strains W50, BE1 and BR1.
Molecular basis for avirulence of spontaneous variants of Porphyromonas gingivalis: Genomic analysis of strains W50, BE1 and BR1.
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DOI：
10.1111/omi.12373
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发表时间：
2022-06
影响因子：
3.7
通讯作者：
Curtis, Michael A.
中科院分区：
文献类型：
--
作者：
Aduse-Opoku, Joseph;Joseph, Susan;Devine, Deirdre A.;Marsh, Philip D.;Curtis, Michael A.
牙周病原体牙龈卟啉单胞菌是遗传异质性。然而，也报告了表型不同的亚株的自发产生。McKee等人（1988）在研究牙龈卟啉单胞菌W50的生长和特性期间，在恒化器中培养该细菌。血琼脂平板上的细胞活力显示，第7天后在高氯高铁血红素培养基中生长的样本中存在两种类型的非着色变体，W50米色（BE 1）和W50棕色（BR 1），到第21天，这些变体的数量增加至约占总计数的25%。W50，BE 1和BR 1的表型改变色素沉着，蛋白酶活性和血凝和敏感性降低补体杀伤。此外，变体在小鼠毒力模型中表现出显著的减毒。其他研究者表明，在BE 1中，主要的细胞外精氨酸牙龈卟啉菌蛋白酶是RgpB，并且与A脂多糖特异性MAb 1B 5没有反应（Collinson et al.，1998; Slaney等人，2006年）。为了确定这些表型特性的遗传基础，我们使用Oxford Nanopore和Illumina HiSeq平台的短配对末端DNA序列读取进行了杂交DNA序列长读取，以生成亲本和变体的闭合环状基因组。比较分析表明，在两种变体BE 1和BR 1中，hagA‐kgp基因座的20 kb区域中缺失完整的kgp。hagA的缺失导致变异体中的开放阅读框变小，BR 1发生了主要的染色体DNA倒位。还观察到两种变体的基因组的其他微小变化。鉴于Kgp和HagA的蛋白酶活性和血凝的重要性，分别在这种细菌中，在这个位点的基因组变化可能占大多数的变异体的表型改变。hagA和kgp的同源性和重复性以及反向连接处的特征表明了特异性和稳定的同源重组事件，这可能是该物种遗传异质性的基础。
The periodontal pathogen Porphyromonas gingivalis is genetically heterogeneous. However, the spontaneous generation of phenotypically different sub‐strains has also been reported. McKee et al. (1988) cultured P. gingivalis W50 in a chemostat during investigations into the growth and properties of this bacterium. Cell viability on blood agar plates revealed two types of non‐pigmenting variants, W50 beige (BE1), and W50 brown (BR1), in samples grown in a high‐hemin medium after day 7, and the population of these variants increased to approximately 25% of the total counts by day 21. W50, BE1 and BR1 had phenotypic alterations in pigmentation, reduced protease activity and haemagglutination and susceptibility to complement killing. Furthermore, the variants exhibited significant attenuation in a mouse model of virulence. Other investigators showed that in BE1, the predominant extracellular Arg‐gingipain was RgpB, and no reaction with an A‐lipopolysaccharide‐specific MAb 1B5 (Collinson et al., 1998; Slaney et al., 2006). In order to determine the genetic basis for these phenotypic properties, we performed hybrid DNA sequence long reads using Oxford Nanopore and the short paired‐end DNA sequence reads of Illumina HiSeq platforms to generate closed circular genomes of the parent and variants. Comparative analysis indicated loss of intact kgp in the 20 kb region of the hagA‐kgp locus in the two variants BE1 and BR1. Deletions in hagA led to smaller open reading frames in the variants, and BR1 had incurred a major chromosomal DNA inversion. Additional minor changes to the genomes of both variants were also observed. Given the importance of Kgp and HagA to protease activity and haemagglutination, respectively, in this bacterium, genomic changes at this locus may account for most of the phenotypic alterations of the variants. The homologous and repetitive nature of hagA and kgp and the features at the inverted junctions are indicative of specific and stable homologous recombination events, which may underlie the genetic heterogeneity of this species.
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影响因子：
14.9
作者：
Chen IA;Chu K;Palaniappan K;Pillay M;Ratner A;Huang J;Huntemann M;Varghese N;White JR;Seshadri R;Smirnova T;Kirton E;Jungbluth SP;Woyke T;Eloe-Fadrosh EA;Ivanova NN;Kyrpides NC
通讯作者：
Kyrpides NC
DOI：
10.1128/genomea.00927-15
发表时间：
2015-09-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Chastain-Gross, Ryan P.;Xie, Gary;Progulske-Fox, Ann
通讯作者：
Progulske-Fox, Ann
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ
影响因子：
3.2
作者：
Klein, Brian A.;Cornacchione, Louis P.;Hu, Linden T.
通讯作者：
Hu, Linden T.
影响因子：
4.8
作者：
Glew, Michelle D.;Veith, Paul D.;Reynolds, Eric C.
通讯作者：
Reynolds, Eric C.