Functional metagenomic libraries generated from anthropogenically impacted environments reveal importance of metabolic genes in biocide and antibiotic resistance.
Functional metagenomic libraries generated from anthropogenically impacted environments reveal importance of metabolic genes in biocide and antibiotic resistance.
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从人为影响的环境中产生的功能宏基因组文库揭示了代谢基因在杀菌剂和抗生素耐药性中的重要性。
DOI：
10.1016/j.crmicr.2023.100184
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发表时间：
2023
影响因子：
--
通讯作者：
Gaze, William H.
中科院分区：
文献类型：
--
作者：
Murray, Aimee K.;Zhang, Lihong;Snape, Jason;Gaze, William H.
对三种环境下的功能文库进行了生物杀灭剂抗性筛选。对生物杀灭剂的抗性在纺织废水修复土壤和污水饼中普遍存在。对推定的抗性基因进行了鉴定。大风样基因最常见，并降低了抗生素敏感性。类似大风的基因具有潜在的可调动性。人为活动导致耐药细菌和抗微生物化合物的混合物释放到环境中，这可能直接选择或间接共同选择抗微生物药物耐药性（AMR）。许多研究使用宏基因组测序或基于qpcr的方法来研究环境抗性组，但这些方法受到先验知识的限制。在本研究中，采用功能宏基因组方法探索了两个污染环境和一个原始地点的杀菌剂耐药机制，并确定具有杀菌剂耐药的潜在新基因是否也具有耐药性或降低对抗生素的敏感性。抗性主要是通过新的机制介导的，而不是众所周知的qac外排基因。与敲除突变体相比，在两种污染环境中都鉴定出了udp -半乳糖4- epimase （galE）样基因，并首次显示出对杀菌剂和临床重要抗生素的交叉抗性（据我们所知）。类似GalE的基因也与转座子位于同一位置，表明具有动员潜力。这些结果表明，管家基因可能在环境微生物组的AMR中发挥了重要但未被充分认识的作用。
Functional libraries from three environments were screened for biocide resistance. Biocide resistance was common in textile effluent remediating soil and sewage cake. Putative resistance genes were characterised. GalE like genes were most common and conferred reduced antibiotic susceptibility. GalE like genes were potentially mobilizable. Anthropogenic activities result in the release of antimicrobial resistant bacteria and a cocktail of antimicrobial compounds into the environment that may directly select or indirectly co-select for antimicrobial resistance (AMR). Many studies use metagenome sequencing or qPCR-based approaches to study the environmental resistome but these methods are limited by a priori knowledge. In this study, a functional metagenomic approach was used to explore biocide resistance mechanisms in two contaminated environments and a pristine site, and to identify whether potentially novel genes conferring biocide resistance also conferred resistance or reduced susceptibility to antibiotics. Resistance was predominately mediated through novel mechanisms exclusive of the well-known qac efflux genes. UDP-galactose 4-epimerase (galE) –like genes were identified in both contaminated environments and were shown to confer cross-resistance to biocides and clinically important antibiotics for the first time (to our knowledge), compared to knockout mutants. GalE -like genes were also co-located with transposons, suggesting mobilisation potential. These results show that housekeeping genes may play a significant yet underappreciated role in AMR in environmental microbiomes.
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影响因子：
3.7
作者：
Carlson-Banning KM;Chou A;Liu Z;Hamill RJ;Song Y;Zechiedrich L
通讯作者：
Zechiedrich L
影响因子：
4.9
作者：
Gaze, WH;Abdouslam, N;Wellington, EMH
通讯作者：
Wellington, EMH
DOI：
10.1111/j.1749-6632.1994.tb44256.x
发表时间：
1994-01-01
期刊：
MICROBIAL PATHOGENESIS AND IMMUNE RESPONSE
影响因子：
--
作者：
JENNINGS, MP;VANDERLEY, P;MOXON, ER
通讯作者：
MOXON, ER
影响因子：
3.7
作者：
Fu C;Donovan WP;Shikapwashya-Hasser O;Ye X;Cole RH
通讯作者：
Cole RH
影响因子：
4.9
作者：
Humeniuk, C;Arlet, G;Philippon, A
通讯作者：
Philippon, A