A Novel AtKEA Gene Family, Homolog of Bacterial K+/H+ Antiporters, Plays Potential Roles in K+ Homeostasis and Osmotic Adjustment in Arabidopsis
A Novel AtKEA Gene Family, Homolog of Bacterial K+/H+ Antiporters, Plays Potential Roles in K+ Homeostasis and Osmotic Adjustment in Arabidopsis
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新型 AtKEA 基因家族（细菌 K /H 逆向转运蛋白的同源物）在拟南芥 K 稳态和渗透压调节中发挥潜在作用
DOI：
10.1371/journal.pone.0081463
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发表时间：
2013-11
期刊：
影响因子：
3.7
通讯作者：
Quan-Sheng Qiu
中科院分区：
文献类型：
--
作者：
Sheng Zheng;Ting Pan;Ligang Fan;Quan-Sheng Qiu
AtKEAs是细菌KefB/KefC的同源物，预计在拟南芥中编码K+/H+反转运蛋白。AtKEA家族包含6个基因，在进化图中形成两个亚群：AtKEA1-3和AtKEA4-6。AtKEA1和AtKEA2具有长n端结构域；全长AtKEA1在酵母中无活性。通过在缺乏多个离子载体的酵母突变体中表达AtKEA基因，分析了其转运活性。AtKEAs对高K+和潮霉素B具有抗性，但对盐和Li+胁迫无抗性。AtKEAs在拟南芥茎部和根中均有表达。AtKEA1、-3和-4在低钾胁迫下表达增强，而AtKEA2和AtKEA5在山梨醇和ABA处理下表达增强。然而，在aba2-3突变体中没有观察到AtKEA2和AtKEA5的渗透诱导，这表明ABA调节了它们的渗透反应机制。AtKEAs的表达可能不受SOS通路的调控，因为它们在SOS突变体中的表达不受影响。GFP标记分析表明，AtKEAs在酵母中分布多样。通过稳定转化的幼苗和原生质体中的瞬时表达，证实了AtKEA3的高尔基定位。总的来说，AtKEAs的表达和定位是多样的，可能在拟南芥的K+稳态和渗透调节中发挥作用。
AtKEAs, homologs of bacterial KefB/KefC, are predicted to encode K+/H+ antiporters in Arabidopsis. The AtKEA family contains six genes forming two subgroups in the cladogram: AtKEA1-3 and AtKEA4-6. AtKEA1 and AtKEA2 have a long N-terminal domain; the full-length AtKEA1 was inactive in yeast. The transport activity was analyzed by expressing the AtKEA genes in yeast mutants lacking multiple ion carriers. AtKEAs conferred resistance to high K+ and hygromycin B but not to salt and Li+ stress. AtKEAs expressed in both the shoot and root of Arabidopsis. The expression of AtKEA1, -3 and -4 was enhanced under low K+ stress, whereas AtKEA2 and AtKEA5 were induced by sorbitol and ABA treatments. However, osmotic induction of AtKEA2 and AtKEA5 was not observed in aba2-3 mutants, suggesting an ABA regulated mechanism for their osmotic response. AtKEAs’ expression may not be regulated by the SOS pathway since their expression was not affected in sos mutants. The GFP tagging analysis showed that AtKEAs distributed diversely in yeast. The Golgi localization of AtKEA3 was demonstrated by both the stably transformed seedlings and the transient expression in protoplasts. Overall, AtKEAs expressed and localized diversely, and may play roles in K+ homeostasis and osmotic adjustment in Arabidopsis.
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影响因子：
3.3
作者：
Bowers, K;Levi, BP;Stevens, TH
通讯作者：
Stevens, TH
影响因子：
5.6
作者：
Chanroj S;Wang G;Venema K;Zhang MW;Delwiche CF;Sze H
通讯作者：
Sze H
影响因子：
3.5
作者：
Quintero, FJ;Blatt, MR;Pardo, JM
通讯作者：
Pardo, JM
分类信号，N末端修饰和叶绿体蛋白质组的丰度。
影响因子：
3.7
作者：
Zybailov, Boris;Rutschow, Heidi;Friso, Giulia;Rudella, Andrea;Emanuelsson, Olof;Sun, Qi;van Wijk, Klaas J.
通讯作者：
van Wijk, Klaas J.
影响因子：
7.4
作者：
FANG, ZW;MI, F;BERKOWITZ, GA
通讯作者：
BERKOWITZ, GA