Molecular Cloning and Expression Responses of Jarid2b to High-Temperature Treatment in Nile Tilapia (Oreochromis niloticus).
Molecular Cloning and Expression Responses of Jarid2b to High-Temperature Treatment in Nile Tilapia (Oreochromis niloticus).
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尼罗罗非鱼 (Oreochromis niloticus) Jarid2b 的分子克隆和对高温处理的表达反应
DOI：
10.3390/genes13101719
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发表时间：
2022-09-25
期刊：
影响因子：
3.5
通讯作者：
Zhao, Yan
中科院分区：
文献类型：
--
作者：
Zhou, Min;Yao, Zhilei;Zhao, Min;Fang, Qingfeng;Ji, Xiangshan;Chen, Hongju;Zhao, Yan
尼罗罗非鱼是一种GSD + TE（Genetic Sex Determination + Temperature Effect）鱼类，在临界热敏期（TSP）高温处理可诱导尼罗罗非鱼遗传雌性发生性逆转，脑转录组显示在TSP期间36 °C高温处理12天后Jarid 2（Jumonji and AT-rich domain containing 2）表达上调。研究表明，JARID 2与多梳抑制复合物2（PRC 2）形成复合物，该复合物催化H3 K27 me 3，其与转录抑制强烈相关。在这项研究中，Jarid 2b的克隆和特征在于尼罗罗非鱼，这是高度保守的分析鱼类物种。高温处理12 d后，21 dpf XX遗传雌性性腺中Jarid 2b的表达量上调，达到与雄性相近的水平。对高温处理的类似反应也出现在大脑、心脏、肝脏、肌肉、眼睛和皮肤组织中。有趣的是，Jarid 2b的表达仅响应于高温处理，而不是17α-甲基睾酮（MT）或来曲唑处理;尽管这些处理也可以诱导遗传尼罗罗非鱼雌性的性逆转。进一步的研究表明，Jarid 2b在高温处理后的第8小时迅速响应。考虑到JARID 2可以招募PRC 2并建立H3 K27 me 3，我们推测它可能是通过调节多个性别分化相关基因位点的H3 K27甲基化水平参与尼罗罗非鱼GSD + TE调控的上游基因。
Nile tilapia is a GSD + TE (Genetic Sex Determination + Temperature Effect) fish, and high-temperature treatment during critical thermosensitive periods (TSP) can induce the sex reversal of Nile tilapia genetic females, and brain transcriptomes have revealed the upregulation of Jarid2 (Jumonji and AT-rich domain containing 2) expression after 36 °C high-temperature treatment for 12 days during TSP. It was shown that JARID2 forms a complex with polycomb repressive complex 2 (PRC2) that catalyzed H3K27me3, which was strongly associated with transcriptional repression. In this study, Jarid2b was cloned and characterized in Nile tilapia, which was highly conserved among the analyzed fish species. The expression of Jarid2b was upregulated in the gonad of 21 dpf XX genetic females after 12-day high-temperature treatment and reached a similar level to that of males. Similar responses to high-temperature treatment also appeared in the brain, heart, liver, muscle, eye, and skin tissues. Interestingly, Jarid2b expression was only in response to high-temperature treatment, and not to 17α-methyltestosterone (MT) or letrozole treatments; although, these treatments can also induce the sex reversal of genetic Nile tilapia females. Further studies revealed that Jarid2b responded rapidly at the 8th hour after high-temperature treatment. Considering that JARID2 can recruit PRC2 and establish H3K27me3, we speculated that it might be an upstream gene participating in the regulation of Nile tilapia GSD + TE through regulating the H3K27 methylation level at the locus of many sex differentiation-related genes.
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影响因子：
3.5
作者：
Fellous, Alexandre;Favrel, Pascal;Riviere, Guillaume
通讯作者：
Riviere, Guillaume
DOI：
10.1016/j.cbpc.2006.05.006
发表时间：
2006-09-01
影响因子：
3.9
作者：
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通讯作者：
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作者：
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影响因子：
4.8
作者：
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Sun, Zu-Wen
影响因子：
7.2
作者：
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通讯作者：
Thomashow, MF