Murine chronic lymph node window for longitudinal intravital lymph node imaging.
Murine chronic lymph node window for longitudinal intravital lymph node imaging.
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DOI：
10.1038/nprot.2017.045
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发表时间：
2017-08
期刊：
影响因子：
14.8
通讯作者：
Padera TP
中科院分区：
文献类型：
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作者：
Meijer EFJ;Jeong HS;Pereira ER;Ruggieri TA;Blatter C;Vakoc BJ;Padera TP
小鼠慢性成像窗口已经发展到允许许多不同器官的活体显微镜，并已被证明是在推进我们的正常和疾病过程的知识至关重要。一个允许对淋巴结进行长期活体成像的模型系统，将有助于研究在各种疾病环境下免疫反应产生过程中淋巴结中的细胞行为，以及在癌症小鼠转移性病变形成过程中。我们描述了一种慢性淋巴结窗口（CLNW）手术准备，允许小鼠腹股沟淋巴结的活体成像。CLNW是由钛定制的，并结合了一个标准的盖子。它允许稳定的纵向成像，而不需要连续的手术，同时保留淋巴结血液和淋巴流动。我们还描述了如何构建和使用专门为CLNW设计的成像平台，以防止成像期间（大）旋转变化以及呼吸运动。整个过程每只小鼠大约需要半小时，随后允许对小鼠淋巴结和周围结构进行长达14天的纵向活体成像。成功执行该方案需要小动物手术经验。
Chronic imaging windows in mice have been developed to allow intravital microscopy of many different organs and have proven to be of paramount importance in advancing our knowledge of normal and disease processes. A model system that allows long-term intravital imaging of lymph nodes would facilitate the study of cell behavior in lymph nodes during the generation of immune responses in a variety of disease settings and during the formation of metastatic lesions in cancer-bearing mice. We describe a chronic lymph node window (CLNW) surgical preparation that allows intravital imaging of the inguinal lymph node in mice. The CLNW is custom-made from titanium and incorporates a standard coverslip. It allows stable longitudinal imaging without the need for serial surgeries while preserving lymph node blood and lymph flow. We also describe how to build and use an imaging stage specifically designed for the CLNW to prevent (large) rotational changes as well as respiratory movement during imaging. The entire procedure takes approximately half an hour per mouse, and subsequently allows for longitudinal intravital imaging of the murine lymph node and surrounding structures for up to 14 d. Small-animal surgery experience is required to successfully carry out the protocol.
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影响因子：
48
作者：
Farrar, Matthew J.;Bernstein, Ida M.;Schlafer, Donald H.;Cleland, Thomas A.;Fetcho, Joseph R.;Schaffer, Chris B.
通讯作者：
Schaffer, Chris B.
影响因子：
11.2
作者：
Hoshida, Tohru;Isaka, Naohide;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.
影响因子：
82.9
作者：
Brown, EB;Campbell, RB;Jain, RK
通讯作者：
Jain, RK
影响因子：
10.3
作者：
Jeong, Han-Sin;Jones, Dennis;Padera, Timothy P.
通讯作者：
Padera, Timothy P.
影响因子：
82.9
作者：
通讯作者：
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