Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging.
Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging.
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活体多光子显微镜为健康和疾病提供了强大的机制见解，并已成为现代生物实验室的常用仪器。然而，实现多光子显微镜所需的高数值孔径和外源造影剂限制了研究大量组织体积或长时间重复探测动态变化的能力。在这里，我们引入光频域成像（OFDI）作为一种活体显微镜，它规避了多光子显微镜的技术限制，从而为组织生物学之前未探索的、至关重要的方面提供了前所未有的途径。使用基于 OFDI 的新颖方法和完全内在的对比机制，我们对肿瘤血管生成、淋巴管生成、组织活力以及血管和细胞对治疗的反应进行快速和重复的测量，从而证明 OFDI 在促进生理和病理过程探索以及治疗策略评估方面的潜力。
Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease, and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here, we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, critically important aspects of tissue biology. Using novel OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies.
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影响因子：
8.6
作者：
GAZIT, Y;BERK, DA;JAIN, RK
通讯作者：
JAIN, RK
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35
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Collins, Hazel A.;Khurana, Mamta;Anderson, Harry L.
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Anderson, Harry L.
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Padera, Timothy P.;Kuo, Angera H.;Jain, Rakesh K.
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Jain, Rakesh K.
影响因子：
82.9
作者：
Brown, EB;Campbell, RB;Jain, RK
通讯作者：
Jain, RK
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3.8
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通讯作者：
Hurst, Sawan