Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells.
Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells.
复制标题
芍药苷对溶血磷脂酰胆碱诱导的人脐静脉内皮细胞炎症因子产生的抑制作用。
DOI：
10.3892/ijmm.2012.1211
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
余术宜
中科院分区：
文献类型：
--
作者：
余术宜
溶血磷脂酰胆碱（LPC）通过引发内皮炎症反应在动脉粥样硬化中起重要作用。芍药苷（PEF），分离自芍药的干燥根，已被报道发挥抗炎作用，但确切的机制还不完全清楚。本研究的目的是探讨脉冲电场对LPC诱导的炎症因子产生的抑制作用及其机制。在人脐静脉内皮细胞（HUVEC）中，在暴露于LPC（10 mg/l）24 h前2 h加入不同浓度（1、10或100 µmol/l）的PEF。结果表明，PEF可显著抑制LPC诱导的炎症因子的产生。此外，PEF还能抑制LPC诱导的高迁移率族蛋白B1（HMGB 1）的表达和释放，上调晚期糖基化终产物受体（RECEPTOR）、Toll样受体（TLR）-2和TLR-4的表达，提高核因子-κB（NF-κB）的活性。我们的研究结果表明，PEF通过抑制HMGB 1-κ B/TLR-2/TLR-4-NF-κB途径抑制LPC诱导的炎症因子产生。
Lysophosphatidylcholine (LPC) plays an important role in atherosclerosis through initiation of endothelial inflammation response. Paeoniflorin (PEF), isolated from the dry root of Paeonia, has been reported to exert an anti-inflammatory effect, but the exact mechanism is not fully understood. The aim of this study was to investigate the inhibitory effects of PEF on LPC-induced inflammatory factor production and the underlying mechanisms. In human umbilical vein endothelial cells (HUVECs), different concentrations (1, 10 or 100 µmol/l) of PEF were added 2 h prior to exposure to LPC (10 mg/l) for 24 h. The results showed that PEF significantly inhibited LPC-induced inflammatory factor production. In addition, PEF was also able to suppress the enhanced high mobility group box-1 (HMGB1) expression and release, upregulated expression of receptor for advanced glycation end product (RAGE), Toll-like receptor (TLR)-2 and TLR-4, and increased nuclear factor-κB (NF-κB) activity induced by LPC. Our results suggest that PEF suppresses LPC-induced inflammatory factor production through inhibition of the HMGB1-RAGE/TLR-2/TLR-4-NF-κB pathway.
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影响因子：
29
作者：
Barish GD;Yu RT;Karunasiri MS;Becerra D;Kim J;Tseng TW;Tai LJ;Leblanc M;Diehl C;Cerchietti L;Miller YI;Witztum JL;Melnick AM;Dent AL;Tangirala RK;Evans RM
通讯作者：
Evans RM
影响因子：
2.8
作者：
Luan, Zheng-Gang;Zhang, Hao;Guo, Ren-Xuan
通讯作者：
Guo, Ren-Xuan
影响因子：
4
作者：
C. Schaefer;C. Kuhlmann;Christine Gast;S. Weiterer;Fang Li;A. Most;T. Neumann;Ulrich Backenköhler;H. Tillmanns;B. Waldecker;J. Wiecha;A. Erdoğan
通讯作者：
C. Schaefer;C. Kuhlmann;Christine Gast;S. Weiterer;Fang Li;A. Most;T. Neumann;Ulrich Backenköhler;H. Tillmanns;B. Waldecker;J. Wiecha;A. Erdoğan
影响因子：
1.6
作者：
Koo, Yean Kyoung;Kim, Jeong Mi;Yun-Choi, Hye Sook
通讯作者：
Yun-Choi, Hye Sook
影响因子：
3.4
作者：
Hyun-Ok Yang;W. Ko;Jae Youn Kim;H. Ro
通讯作者：
Hyun-Ok Yang;W. Ko;Jae Youn Kim;H. Ro