Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China.
Taxonomy, virulence determinants and antimicrobial susceptibility of Aeromonas spp. isolated from bacteremia in southeastern China.
复制标题
气单胞菌属的分类学、毒力决定因素和抗菌敏感性。
DOI：
10.1186/s13756-021-00911-0
复制
发表时间：
2021-02-27
影响因子：
5.5
通讯作者：
Zhou T
中科院分区：
文献类型：
--
作者：
Sun Y;Zhao Y;Xu W;Fang R;Wu Q;He H;Xu C;Zhou C;Cao J;Chen L;Zhou T
背景
本研究旨在阐明中国东南部危及生命的菌血症气单胞菌的种类分类、临床表现、毒力基因谱和抗菌药物敏感性。
方法
从2013年至2018年温州某教学医院分离出引起菌血症的气单胞菌临床样本，并进行回顾性队列研究。利用管家基因gyrB对气单胞菌进行种水平鉴定。采用聚合酶链反应（PCR）筛选毒力和耐药相关基因，并采用VITEK 2 Compact全自动微生物鉴定及药敏分析仪进行药敏试验（AST）。
结果
在6年（2013-2018年）期间，共收集了58株从菌血症患者中分离的气单胞菌。58株分离株分属于5个种，其中达卡气单胞菌（Aeromonasdhakensis）为优势种（26/58），其次为维氏气单胞菌（Aeromonasveronii）（13/58）、豚鼠气单胞菌（Aeromonascauses）（10/58）、嗜水气单胞菌（Aeromonasarena）（7/58）和詹氏气单胞菌（Aeromonasjandaei）（2/58）。58例中16例预后不良。预后不良与肝硬化和不适当的经验性抗菌药物治疗显著相关。气单胞菌引起的菌血症进展极快，尤其是在A.感染。毒力基因aer、lip、hlyA、alt、ast和act的检出率分别为24.1%（14/58）、62.1%（36/58）、65.5%（38/58）、58.6%（34/58）、15.5%（9/58）和65.5%（38/58）。药敏试验结果显示，58株分离株中有9株为多重耐药（MDR）菌。在所有9株MDR分离株中鉴定出blaTEM基因。其中blaSHV 4株，blaAQU-1 2株，blaMOX 1株，blaCepH 3株，blaCphA 8株，aac（6′）-Ib-cr 3株。大多数气单胞菌对第三代头孢菌素、氨基糖苷类、氟喹诺酮类和呋喃妥因保持敏感。
结论
气单胞菌属感染，尤其是气单胞菌属感染的流行和危害性。在临床上被低估了。持续监测是必要的，以保持跟踪MDR气单胞菌，由于最近的流行和更有效的措施，需要控制耐药决定因素的传播。
Background
The study aimed to elucidate the species taxonomy, clinical manifestations, virulence gene profiles and antimicrobial susceptibilities of Aeromonas strains isolated from life-threatening bacteremia in southeastern China.
Methods
Clinical samples of Aeromonas causing bacteremia were isolated from a teaching hospital in Wenzhou from 2013 to 2018 and a retrospective cohort study was performed. Aeromonas strains were identified at species level by housekeeping gene gyrB. Virulence and drug resistance-associated genes were screened by polymerase chain reaction (PCR) and antimicrobial susceptibility testing (AST) was performed by the VITEK 2 Compact system.
Results
A total of 58 Aeromonas isolated from patients with bacteremia were collected during 6 years (2013–2018). 58 isolates were identified to five different species, where Aeromonas dhakensis appeared to be the predominant species (26/58), followed by Aeromonas veronii (13/58), Aeromonas caviae (10/58), Aeromonas hydrophila (7/58) and Aeromonas jandaei (2/58). 16 of 58 patients had poor prognosis. Poor prognosis was significantly associated with liver cirrhosis and inappropriate empirical antimicrobials therapy. The progression of bacteremia caused by Aeromonas was extremely fast, especially in A. dhakensis infections. Virulence genes aer, lip, hlyA, alt, ast, and act, were detected at ratios of 24.1% (14/58), 62.1% (36/58), 65.5% (38/58), 58.6% (34/58), 15.5% (9/58) and 65.5% (38/58), respectively. Antimicrobial susceptibility testing exhibited that 9 out of 58 isolates were identified as multi-drug resistant (MDR) organism. The blaTEM gene was identified in all 9 MDR isolates. blaSHV, blaAQU-1, blaMOX, blaCepH, blaCphA and aac(6′)-Ib-cr were detected in 4 isolates, 2 isolates, 1 isolate, 3 isolates, 8 isolates, and 3 isolates, respectively. The majority of Aeromonas strains maintained susceptible to 3rd generation cephalosporins, aminoglycosides, fluoroquinolones and furantoin.
Conclusions
The prevalence and dangerousness of Aeromonas infections, especially A. dhakensis, are underestimated in clinic. Continuous monitoring is essential to keep track of MDR Aeromonas due to the increasing prevalence recently and a more effective measure is required to control the spread of resistance determinants.
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影响因子：
1.2
作者：
Lay, Chorng-Jang;Zhuang, Han-Juan;Tsai, Chen-Chi
通讯作者：
Tsai, Chen-Chi
影响因子：
2.6
作者：
Segatore, Bernardetta;Piccirilli, Alessandra;Amicosante, Gianfranco
通讯作者：
Amicosante, Gianfranco
影响因子：
5.2
作者：
Chen PL;Lamy B;Ko WC
通讯作者：
Ko WC
影响因子：
2.2
作者：
Kitagawa, Hiroki;Ohge, Hiroki;Sugai, Motoyuki
通讯作者：
Sugai, Motoyuki
DOI：
10.1016/j.ijantimicag.2013.08.002
发表时间：
2013-11-01
影响因子：
10.8
作者：
Wu, Chi-Jung;Wang, Hsuan-Chen;Ko, Wen-Chien
通讯作者：
Ko, Wen-Chien