Lead (Pb) accumulation, oxidative stress and DNA damage induced by dietary Pb in tilapia (Oreochromis niloticus)
Lead (Pb) accumulation, oxidative stress and DNA damage induced by dietary Pb in tilapia (Oreochromis niloticus)
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罗非鱼 (Oreochromis niloticus) 中膳食 Pb 诱导的铅 (Pb) 积累、氧化应激和 DNA 损伤
DOI：
10.1111/j.1365-2109.2011.02817.x
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发表时间：
2012
影响因子：
2
通讯作者：
Xu, Zirong
中科院分区：
文献类型：
--
作者：
Dai, Wei;Liu, Shuxi;Fu, Linglin;Du, Huahua;Xu, Zirong
随着水产养殖中饲料铅污染的增加，需要更好地了解饲料铅对水生动物的毒性效应。将罗非鱼(Oreochromis Niloticus)暴露于0、10 0、4 0 0和80 0μg g−1干重的饲料中60d，研究铅在组织和血液中的积累、后肾的氧化应激和外周血细胞的DNA损伤。结果表明，饲料铅暴露导致组织和血液中铅的显著积累，并随着饲料中铅浓度的增加而增加。铅在采样组织中的累积顺序如下：后部kidney>；bone>；liver>；gill>；spleen>；testis>；muscle>；brain.与对照组相比，饲粮铅显著提高了仔猪体内丙二醛水平，降低了总抗氧化能力(P<；0.05)，并使谷胱甘肽含量、谷胱甘肽过氧化物酶和超氧化物歧化酶活性呈浓度依赖性下降。饲料铅对罗非鱼外周血细胞DNA的损伤呈剂量依赖性。结果表明，饲料铅暴露可导致罗非鱼组织和血液中铅的显著积累，继而引起后肾的氧化应激和外周血细胞的DNA损伤。
With the increasing occurrence of dietary lead (Pb) contamination in aquaculture, a better understanding of the toxic effects of dietary Pb on aquatic animals is needed. Tilapia (Oreochromis niloticus) were exposed to dietary Pb at concentrations of 0, 100, 400 and 800 μg g−1 dry weight for 60 days, and Pb accumulation in tissues and blood, oxidative stress in posterior kidney and DNA damage in peripheral blood cells were investigated. The results showed that dietary Pb exposure resulted in significant Pb accumulation in tissues and blood, which increased with the dietary Pb concentrations. Pb accumulated in sampled tissues in the following order: posterior kidney>bone>liver>gill>spleen>testis>muscle>brain. Dietary Pb caused a significant increase in the malondialdehyde level and a significant decrease in the total antioxidant capacity content when compared with the control group (P<0.05), accompanied by concentration-dependent decreases in the glutathione content, glutathione peroxidase and superoxide dismutase activities. Pb dose-dependent DNA damage was observed in peripheral blood cells of tilapia exposed to dietary Pb. The results suggest that dietary Pb exposure can induce significant Pb accumulation in tissues and blood, followed by oxidative stress in posterior kidney and DNA damage in peripheral blood cells of tilapia.
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影响因子：
4.5
作者：
Patlolla, Anita K.;Barnes, Constance;Yedjou, Clement;Velma, V. R.;Tchounwou, Paul B.
通讯作者：
Tchounwou, Paul B.
DOI：
10.1007/s00244-005-0212-7
发表时间：
2006-11-01
影响因子：
4
作者：
Alves, L. C.;Glover, C. N.;Wood, C. M.
通讯作者：
Wood, C. M.
影响因子：
4.5
作者：
A. Ojo;C. Wood
通讯作者：
A. Ojo;C. Wood
DOI：
10.1016/s1383-5718(02)00295-4
发表时间：
2003-03
期刊：
Mutation research
影响因子：
--
作者：
K. Woźniak;J. Błasiak
通讯作者：
K. Woźniak;J. Błasiak
影响因子：
4.5
作者：
C. Trenzado;M. Hidalgo;M. García-Gallego;A. E. Morales;M. Furné;A. Domezain;J. Domezain;A. Sanz
通讯作者：
C. Trenzado;M. Hidalgo;M. García-Gallego;A. E. Morales;M. Furné;A. Domezain;J. Domezain;A. Sanz