Mapping precursor movement through the postnatal thymus reveals specific microenvironments supporting defined stages of early lymphoid development.
Mapping precursor movement through the postnatal thymus reveals specific microenvironments supporting defined stages of early lymphoid development.
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DOI：
10.1084/jem.194.2.127
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发表时间：
2001-07-16
期刊：
影响因子：
--
通讯作者：
Petrie HT
中科院分区：
文献类型：
--
作者：
Lind EF;Prockop SE;Porritt HE;Petrie HT
细胞分化是一个复杂的过程，涉及谱系规范、增殖、功能能力的赋予以及存活或细胞死亡的整合信号。在胚胎发生过程中，在组织块的生长过程中建立了调节这些过程的空间离散环境，该过程也导致发育事件的时间分离。在经历稳态产后分化的组织中，必须建立另一种诱导发育线索的空间和时间分离的方法。在这里，我们表明，在出生后的胸腺中，这是通过诱导血源性前体进入髓周皮质狭窄区域的器官，然后穿过皮质向外迁移，然后积聚在被膜下区域来实现的。值得注意的是，血液前体不会在未分化状态下迁移到皮质，而是在此过程中经历渐进的发育变化，使得特定的前体阶段出现在不同的皮质区域中。这些皮质区域的识别，加上有关相应淋巴前体遗传潜力的现有知识，为可能诱发这些分化事件的基质环境设定了操作界限。我们得出的结论是，形态相似但功能不同的基质区域之间的活跃细胞迁移是调节稳态胸腺分化和稳态的重要组成部分。
Cellular differentiation is a complex process involving integrated signals for lineage specification, proliferation, endowment of functional capacity, and survival or cell death. During embryogenesis, spatially discrete environments regulating these processes are established during the growth of tissue mass, a process that also results in temporal separation of developmental events. In tissues that undergo steady-state postnatal differentiation, another means for inducing spatial and temporal separation of developmental cues must be established. Here we show that in the postnatal thymus, this is achieved by inducing blood-borne precursors to enter the organ in a narrow region of the perimedullary cortex, followed by outward migration across the cortex before accumulation in the subcapsular zone. Notably, blood precursors do not transmigrate the cortex in an undifferentiated state, but rather undergo progressive developmental changes during this process, such that defined precursor stages appear in distinct cortical regions. Identification of these cortical regions, together with existing knowledge regarding the genetic potential of the corresponding lymphoid precursors, sets operational boundaries for stromal environments that are likely to induce these differentiative events. We conclude that active cell migration between morphologically similar but functionally distinct stromal regions is an integral component regulating differentiation and homeostasis in the steady-state thymus.
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影响因子：
4.4
作者：
Anderson, M;Anderson, SK;Farr, AG
通讯作者：
Farr, AG
影响因子：
64.8
作者：
ARDAVIN, C;WU, L;SHORTMAN, K
通讯作者：
SHORTMAN, K
影响因子：
10.5
作者：
Hoffman, ES;Passoni, L;Hayday, AC
通讯作者：
Hayday, AC
DOI：
10.1073/pnas.95.5.2486
发表时间：
1998-03-03
影响因子：
11.1
作者：
Akashi, K;Kondo, M;Weissman, IL
通讯作者：
Weissman, IL
DOI：
10.1073/pnas.86.5.1614
发表时间：
1989-03-01
影响因子：
11.1
作者：
PEARSE, M;WU, L;SCOLLAY, R
通讯作者：
SCOLLAY, R