Differential expression profiling of head and neck squamous cell carcinoma (HNSCC).
Differential expression profiling of head and neck squamous cell carcinoma (HNSCC).
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DOI：
10.1038/sj.bjc.6601373
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发表时间：
2003-11-17
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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作者：
头颈部鳞状细胞癌（HNSCC）是男性第五大常见癌症，全球每年新发病例约78万例，生存率低。有必要更好地了解HNSCC，制定合理的靶向干预措施，并确定新的预后或诊断标志物。为了解决这些需求，我们进行了大规模的差异显示比较下咽HNSCCs对组织学正常组织从相同的患者。我们已经确定了70个基因，这些基因在肿瘤和正常组织之间的表达存在显著差异。与使用其他技术和更异质的肿瘤样本的其他HNSCC基因表达研究只有有限的重叠。我们的研究结果为HNSCC的理解提供了新的见解。在基因组水平上，一系列差异表达基因聚集在12 p12 -13和1 q21这两个基因组破坏的热点区域。已知的基因在角质形成细胞分化、血管生成、免疫、解毒和细胞表面受体中共享功能关系。特别令人感兴趣的是13个“未知”的基因，只存在于EST，理论上的cDNA和蛋白质数据库，或作为染色体位置。我们发现的差异表达基因是潜在的新标记物和治疗靶点。
Head and neck squamous cell carcinoma (HNSCC) is the fifth most common cancer in men with an incidence of about 780 000 new cases per year worldwide and a poor rate of survival. There is a need for a better understanding of HNSCC, for the development of rational targeted interventions and to define new prognostic or diagnostic markers. To address these needs, we performed a large-scale differential display comparison of hypopharyngeal HNSCCs against histologically normal tissue from the same patients. We have identified 70 genes that exhibit a striking difference in expression between tumours and normal tissues. There is only a limited overlap with other HNSCC gene expression studies that have used other techniques and more heterogeneous tumour samples. Our results provide new insights into the understanding of HNSCC. At the genome level, a series of differentially expressed genes cluster at 12p12–13 and 1q21, two hotspots of genome disruption. The known genes share functional relationships in keratinocyte differentiation, angiogenesis, immunology, detoxification, and cell surface receptors. Of particular interest are the 13 ‘unknown’ genes that exist only in EST, theoretical cDNA and protein databases, or as chromosomal locations. The differentially expressed genes that we have identified are potential new markers and therapeutic targets.
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影响因子：
10.5
作者：
Coussens, LM;Raymond, WW;Hanahan, D
通讯作者：
Hanahan, D
影响因子：
2.9
作者：
Connolly, GP;Duley, JA;Stacey, NC
通讯作者：
Stacey, NC
影响因子：
8
作者：
El-Naggar, AK;Kim, HW;Lee, JJ
通讯作者：
Lee, JJ
影响因子：
5
作者：
Bar-Eli, M
通讯作者：
Bar-Eli, M
DOI：
10.1016/s0167-4781(00)00196-2
发表时间：
2000-10-02
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
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作者：
Bingle, CD;Bingle, L
通讯作者：
Bingle, L